Nanostructured recombinant protein particles raise specific antibodies against the nodavirus NNV coat protein in sole
Thwaite, Rosemary (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Berbel, Concepción (Instituto de Investigación y Formación Agraria y Pesquera (IFAPA). Centro "El Toruño")
Aparicio, Manuel (Instituto de Investigación y Formación Agraria y Pesquera (IFAPA). Centro "El Toruño")
Torrealba, Debora (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Pesarrodona Roches, Mireia (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Borrego, Juan José (Universidad de Málaga. Departamento de Microbiología)
Manchado, Manuel (Instituto de Investigación y Formación Agraria y Pesquera (IFAPA). Centro "El Toruño")
Roher Armentia, Nerea (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia

Date:	2020
Abstract:	Nervous necrosis virus (NNV) reassortant strains RGNNV/SJNNV have emerged as a potent threat to the Mediterranean marine aquaculture industry, causing viral encephalopathy and retinopathy (VER) in Senegalese sole (Solea senegalensis). In this study, a cheap and practical vaccine strategy using bacterial inclusion bodies made of the coat protein of a virulent reassortant strain of this betanodavirus was devised. The nanostructured recombinant protein nanoparticles, VNNV-C, were administered without adjuvant to two groups of juvenile sole, one by intraperitoneal injection and the other by oral intubation. Specific antibodies were raised in vivo against the NNV coat protein via both routes, with a substantial specific antibody expansion in the injected group 30 days post homologous prime boost. Expression levels of five adaptive immune-related genes, cd8a, cd4, igm, igt and arg2, were also quantified in intestine, spleen and head kidney. Results showed cd4 and igm were upregulated in the head kidney of injected fish, indicating activation of an adaptive systemic response, while intubated fish exhibited a mucosal response in the intestine. Neither route showed significant differential expression of cd8a. The specific antibody response elicited in vivo and the lack of any signs of toxicity over the 6-week study period in young fish (n = 100), evidences the potential of the nanoparticle as a vaccine candidate.
Grants:	Ministerio de Economía y Competitividad AGL2015-65129-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-345 Ministerio de Economía y Competitividad AGL2014-53190-REDC
Note:	Altres ajuts: Fondo Europeo Marítimo y de la Pesca 2014-2020 and IFAPA project PP.FEM.PPA201700.15
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	NNV ; Protein nanoparticles ; Vaccine ; Oral gavage ; Antibodies ; Mucosal immunity ; Viral antigens
Published in:	Fish & shellfish immunology, Vol. 99 (April 2020) , p. 578-586, ISSN 1095-9947

DOI: 10.1016/j.fsi.2020.02.029
PMID: 32105827

Article. Postprint 19 p, 527.1 KB	Material acompanyament 6 p, 959.6 KB	Figura 1. Arxiu tif 2.0 MB	Figura 2. Arxiu tif 2.5 MB	Figura 3. Arxiu tif 685.1 KB	Figura 4. Arxiu tif 257.4 KB
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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
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