Assessing the Diversity and Population Substructure of Sarda Breed Bucks by Using Mtdna and Y-Chromosome Markers
Dettori, Maria Luisa (University of Sassari. Department of Veterinary Medicine)
Petretto, Elena (University of Sassari. Department of Veterinary Medicine)
Pazzola, Michele (University of Sassari. Department of Veterinary Medicine)
Vidal, Oriol (Universitat de Girona. Departament de Biologia)
Amills i Eras, Marcel (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Vacca, Giuseppe Massimo (University of Sassari. Department of Veterinary Medicine)

Data:	2020
Resum:	A sample of 146 Sarda bucks from eight subregions of Sardinia, Italy (Nuorese, Barbagia, Baronia, Ogliastra, Sarrabus, Guspinese, Iglesiente, Sulcis) were characterized for Y-chromosome and mtDNA markers to assess the levels of population substructure. Five polymorphic loci (SRY, AMELY, ZFY, and DDX3Y) on the Y-chromosome were genotyped. The control region of mtDNA was sequenced as a source of complementary information. Analysis of Y-chromosome data revealed the segregation of 5 haplotypes: Y1A (66. 43%), Y2 (28. 57%), Y1C (3. 57%), Y1B1 (0. 71%), and Y1B2 (0. 71%). High levels of Y-chromosome diversity were observed in populations from Southwest Sardinia. The FST values based on Y-chromosome and mtDNA data were low, although a paternal genetic differentiation was observed when comparing the Nuorese and Barbagia populations (Central Sardinia) with the Sulcis, Iglesiente, and Sarrabus populations (Southern Sardinia). AMOVA analysis supported the lack of population substructure. These results suggest the occurrence of a historical and extensive gene flow between Sarda goat populations from different locations of Sardinia, despite the fact that this island is covered by several large mountain ranges. Introgression with foreign caprine breeds in order to improve milk production might have also contributed to avoiding the genetic differentiation amongst Sarda populations.
Nota:	Altres ajuts: CUPJ73C17000070007
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Y-chromosome ; Mitochondrial DNA ; Buck ; Population structuring ; Goat ; SRY ; AMELY ; ZFY ; DDX3Y
Publicat a:	Animals, Vol. 10 Núm. 12 (2020) , p. 2194, ISSN 2076-2615

DOI: 10.3390/ani10122194
PMID: 33255190

13 p, 1.3 MB

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