Lab in a tube : point-of-care detection of Escherichia coli
Amin, Niloufar (Institut Català de Nanociència i Nanotecnologia)
Sena Torralba, Amadeo (Institut Català de Nanociència i Nanotecnologia)
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia)
Afkhami, Abbas (Bu-Ali Sina University. Faculty of Chemistry)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Data:	2020
Resum:	Significant levels of infectious diseases caused by pathogenic bacteria are nowadays a worldwide matter, carrying considerable public health care challenges and huge economic concerns. Because of the rapid transmission of these biothreat agents and the outbreak of diseases, a rapid detection of pathogens in early stages is crucial, particularly in low-resources settings. To this aim, we developed for the first time a new sensing approach carried out in a single step for Escherichia coli O157:H7 detection. The detection principle is based on Förster resonance energy transfer using gold nanoclusters as a signal reporter and gold nanoparticles conjugated with antibodies as a quencher. The sensing platform includes an ultraviolet-light-emitting diode to provide the proper excitation and consists of a microtube containing two pieces of fiber glass; one of them is embedded with label-free gold nanoclusters and the other one with gold nanoparticles conjugated with antibodies. Upon the addition of the sample containing bacteria, the florescence of gold nanoclusters is recovered. The assay was evaluated by the naked eye (on/off) and quantitatively with use of a smartphone camera. The biosensor proved to be highly specific and sensitive, achieving a limit of detection as low as 4. 0 cfu mL. Additionally, recoveries of 110% and 95% were obtained when the platforms in spiked river and tap water, respectively, were evaluated.
Ajuts:	Ministerio de Economía y Competitividad MAT2017-87202-P Ministerio de Economía y Competitividad SEV-2017-0706
Nota:	Altres ajuts: the ICN2 is funded by the CERCA Programme/Generalitat de Catalunya. N.A. acknowledges the Food and Drug Laboratory Research Center, Food and Drug Organization of Iran, and Ministry of Science, Research and Technology of Iran for scholarships during this research time. A.S.T. acknowledges Universitat Autònoma de Barcelona for the possibility of carrying out this work inside the framework of its PhD Programme in Biotechnology.
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Escherichia coli O157:H7 ; Infectious disease ; Pathogenic bacterium ; Proper excitations ; Rapid transmission ; Resonance energy transfer ; Smart-phone cameras ; Ultraviolet light emitting diodes
Publicat a:	Analytical chemistry, Vol. 92, issue 6 (March 2020) , p. 4209-4216, ISSN 1520-6882

DOI: 10.1021/acs.analchem.9b04369

Postprint 10 p, 1.1 MB

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