KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
Tondo, Maria Laura (Universidad Nacional de Rosario. Área Biología Molecular)
de Pedro Jové, Roger (Centre de Recerca en Agrigenòmica)
Vandecaveye, Agustina (Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (Argentina))
Piskulic, Laura (Universidad Nacional de Rosario. Área Estadística y Procesamiento de Datos (Argentina))
Orellano, Elena G. (Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (Argentina))
Valls, Marc (Centre de Recerca en Agrigenòmica)

Date:	2020
Abstract:	Ralstonia solanacearum is the causative agent of bacterial wilt disease on a wide range of plant species. Besides the numerous bacterial activities required for host invasion, those involved in the adaptation to the plant environment are key for the success of infection. R. solanacearum ability to cope with the oxidative burst produced by the plant is likely one of the activities required to grow parasitically. Among the multiple reactive oxygen species (ROS)-scavenging enzymes predicted in the R. solanacearum GMI1000 genome, a single monofunctional catalase (KatE) and two KatG bifunctional catalases were identified. In this work, we show that these catalase activities are active in bacterial protein extracts and demonstrate by gene disruption and mutant complementation that the monofunctional catalase activity is encoded by katE. Different strategies were used to evaluate the role of KatE in bacterial physiology and during the infection process that causes bacterial wilt. We show that the activity of the enzyme is maximal during exponential growth in vitro and this growth-phase regulation occurs at the transcriptional level. Our studies also demonstrate that katE expression is transcriptionally activated by HrpG, a central regulator of R. solanacearum induced upon contact with the plant cells. In addition, we reveal that even though both KatE and KatG catalase activities are induced upon hydrogen peroxide treatment, KatE has a major effect on bacterial survival under oxidative stress conditions and especially in the adaptive response of R. solanacearum to this oxidant. The katE mutant strain also exhibited differences in the structural characteristics of the biofilms developed on an abiotic surface in comparison to wild-type cells, but not in the overall amount of biofilm production. The role of catalase KatE during the interaction with its host plant tomato is also studied, revealing that disruption of this gene has no effect on R. solanacearum virulence or bacterial growth in leave tissues, which suggests a minor role for this catalase in bacterial fitness in planta. Our work provides the first characterization of the R. solanacearum catalases and identifies KatE as a bona fide monofunctional catalase with an important role in bacterial protection against oxidative stress.
Grants:	Agencia Estatal de Investigación AGL2016-78002-R Agencia Estatal de Investigación PID2019-108595RB-I00 Ministerio de Economía y Competitividad SEV/2015/0533
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Ralstonia solanacearum ; Bacterial wilt ; Oxidative burst ; KatE catalase ; Host adaptation
Published in:	Frontiers in plant science, Vol. 11 (July 2020) , art. 1156, ISSN 1664-462X

DOI: 10.3389/fpls.2020.01156
PMID: 32849714

12 p, 1.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
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