A qPCR-based method for the detection and quantification of the peach powdery mildew (Podosphaera pannosa) in epidemiological studies
Marimon, Neus (Centre de Recerca en Agrigenòmica)
Eduardo, Iban (Centre de Recerca en Agrigenòmica)
León, Maela (Universitat Politècnica de València. Instituto Agroforestal Mediterráneo)
Berbegal Martínez, Mónica (Universitat Politècnica de València. Instituto Agroforestal Mediterráneo)
Armengol, Josep (Universitat Politècnica de València. Instituto Agroforestal Mediterráneo)
Luque, Jordi (Institut de Recerca i Tecnologia Agroalimentàries. Patologia vegetal)

Data:	2020
Resum:	A qPCR-based method was developed to detect and quantify Podosphaera pannosa, the main causal agent of peach powdery mildew. A primer pair was designed to target part of the ITS region of the fungal ribosomal DNA, which proved to be highly specific and sensitive. A minimum of 2. 81 pg µL− 1 of P. pannosa DNA and 6 conidia mL− 1 in artificially-prepared conidia suspensions were found to be the limit of detection. Moreover, a quantification of conidia placed on plastic tapes commonly used in volumetric air samplers was performed. Regression equations on conidia quantification obtained either from aqueous conidia suspensions or conidia placed on plastic tapes were similar. The protocol was further validated in field conditions by estimating the number of P. pannosa conidia obtained with an air sampler, by both microscopic and molecular quantification. Both techniques detected the peaks of conidia production during a 4-month sampling period, and a significant correlation (r = 0. 772) was observed between both quantification methods. Additionally, the molecular method was applied to detect latent fungal inoculum in different plant parts of peach trees. The pathogen was detected mainly on the bark of affected twigs, and to a lesser extent, in foliar buds. The method developed here can be applied in the study of P. pannosa epidemiology and can help in improving the management of this pathogen through its early detection and quantification.
Ajuts:	Ministerio de Economía y Competitividad RTA2013-00004-C03-01
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya and INIA with a predoctoral grant (CPD-2015-0142)
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Aerobiology ; Epidemiology ; Molecular technique ; Powdery mildew ; Prunus persica
Publicat a:	European Journal of Plant Pathology, Vol. 158, Issue 4 (December 2020) , p. 1005-1016, ISSN 0929-1873

DOI: 10.1007/s10658-020-02136-0

Postprint 34 p, 732.9 KB

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