Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant Arabidopsis thaliana under Bicarbonate Exposure
Pérez-Martín, Laura (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)
Poschenrieder, Charlotte (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)
Busoms, Silvia (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)
Tolrà Pérez, Roser (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)

Data:	2021
Resum:	High bicarbonate concentrations of calcareous soils with high pH can affect crop performance due to different constraints. Among these, Fe deficiency has mostly been studied. The ability to mobilize sparingly soluble Fe is a key factor for tolerance. Here, a comparative transcriptomic analysis was performed with two naturally selected Arabidopsis thaliana demes, the carbonate-tolerant A1(c+) and the sensitive T6(c−). Analyses of plants exposed to either pH stress alone (pH 5. 9 vs. pH 8. 3) or to alkalinity caused by 10 mM NaHCO3 (pH 8. 3) confirmed better growth and nutrient homeostasis of A1(c+) under alkaline conditions. RNA-sequencing (RNA-seq) revealed that bicarbonate quickly (3 h) induced Fe deficiency-related genes in T6(c−) leaves. Contrastingly, in A1(c+), initial changes concerned receptor-like proteins (RLP), jasmonate (JA) and salicylate (SA) pathways, methionine-derived glucosinolates (GS), sulfur starvation, starch degradation, and cell cycle. Our results suggest that leaves of carbonate-tolerant plants do not sense iron deficiency as fast as sensitive ones. This is in line with a more efficient Fe translocation to aerial parts. In A1(c+) leaves, the activation of other genes related to stress perception, signal transduction, GS, sulfur acquisition, and cell cycle precedes the induction of iron homeostasis mechanisms yielding an efficient response to bicarbonate stress.
Ajuts:	Agencia Estatal de Investigación PID2019-104000RB-I00 Ministerio de Economía, Industria y Competitividad BFU2016-75176-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Transcriptomic ; Arabidopsis thaliana ; Alkaline stress ; Glucosinolate ; Salicylate
Publicat a:	International journal of molecular sciences, Vol. 22, Issue 3 (February 2021) , art. 1226, ISSN 1422-0067

DOI: 10.3390/ijms22031226
PMID: 33513755

24 p, 3.8 MB

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