Human sperm displays rapid responses to diet
Nätt, D. (Linköping University. Department of Clinical and Experimental Medicine. Division of Neurobiology)
Kugelberg, U. (Linköping University. Department of Clinical and Experimental Medicine. Division of Neurobiology)
Casas Masnou, Eduard (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Nedstrand, E. (Linköping University. Department of Clinical and Experimental Medicine. Division of Obstetrics and Gynecology)
Zalavary, S. (Linköping University. Department of Clinical and Experimental Medicine. Division of Obstetrics and Gynecology)
Henriksson, P. (Linköping University. Department of Medical and Health Sciences. Division of Community Medicine)
Nijm, C. (Linköping University. Department of Clinical and Experimental Medicine. Division of Obstetrics and Gynecology)
Jäderquist, J. (Linköping University. Department of Clinical and Experimental Medicine. Division of Obstetrics and Gynecology)
Sandborg, J. (Linköping University. Department of Medical and Health Sciences. Division of Community Medicine)
Flinke, E. (Linköping University. Department of Medical and Health Sciences. Division of Community Medicine)
Ramesh, Ramamoorthy (Linköping University. Department of Clinical and Experimental Medicine. Division of Neurobiology)
Örkenby, L. (Linköping University. Department of Clinical and Experimental Medicine. Division of Neurobiology)
Appelkvist, F. (Linköping University. Department of Clinical and Experimental Medicine. Division of Neurobiology)
Lingg, T. (Linköping University. Department of Clinical and Experimental Medicine. Division of Neurobiology)
Guzzi, N. (Lund University. Stem Cell Center. Department of Laboratory Medicine. Division of Molecular Hematology)
Bellodi, C. (Lund University. Stem Cell Center. Department of Laboratory Medicine. Division of Molecular Hematology)
Löf, M. (Linköping University. Department of Medical and Health Sciences. Division of Community Medicine)
Vavouri, Tanya (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Öst, A. (Linköping University. Department of Clinical and Experimental Medicine. Division of Neurobiology)
Universitat Autònoma de Barcelona

Date:	2019
Abstract:	The global rise in obesity and steady decline in sperm quality are two alarming trends that have emerged during recent decades. In parallel, evidence from model organisms shows that paternal diet can affect offspring metabolic health in a process involving sperm tRNA-derived small RNA (tsRNA). Here, we report that human sperm are acutely sensitive to nutrient flux, both in terms of sperm motility and changes in sperm tsRNA. Over the course of a 2-week diet intervention, in which we first introduced a healthy diet followed by a diet rich in sugar, sperm motility increased and stabilized at high levels. Small RNA-seq on repeatedly sampled sperm from the same individuals revealed that tsRNAs were up-regulated by eating a high-sugar diet for just 1 week. Unsupervised clustering identified two independent pathways for the biogenesis of these tsRNAs: one involving a novel class of fragments with specific cleavage in the T-loop of mature nuclear tRNAs and the other exclusively involving mitochondrial tsRNAs. Mitochondrial involvement was further supported by a similar up-regulation of mitochondrial rRNA-derived small RNA (rsRNA). Notably, the changes in sugar-sensitive tsRNA were positively associated with simultaneous changes in sperm motility and negatively associated with obesity in an independent clinical cohort. This rapid response to a dietary intervention on tsRNA in human sperm is attuned with the paternal intergenerational metabolic responses found in model organisms. More importantly, our findings suggest shared diet-sensitive mechanisms between sperm motility and the biogenesis of tsRNA, which provide novel insights about the interplay between nutrition and male reproductive health.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Adult ; Diet ; Humans ; Male ; Obesity ; RNA ; RNA, Transfer ; Sperm Motility ; Spermatozoa
Published in:	PLoS biology, Vol. 17 Núm. 12 (2019) , p. e3000559, ISSN 1545-7885

DOI: 10.1371/journal.pbio.3000559
PMID: 31877125

25 p, 5.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP) > Josep Carreras Leukaemia Research Institute
Articles > Research articles
Articles > Published articles