Nuclear RNA purification by flow cytometry to study nuclear processes in plants
Moro, Belen (Centre de Recerca en Agrigenòmica)
Kisielow, Malgorzata (Zurich Flow Cytomtery Core Facility)
Barragan Borrero, Veronica (Gregor Mendel Institute of Molecular Plant Biology)
Bouet, Antoine (Centre de Recerca en Agrigenòmica)
Brosnan, Christopher (University of Queensland, Brisbane)
Bologna, Nicolás (Centre de Recerca en Agrigenòmica)

Date:	2021
Abstract:	The nature of plant tissues has continuously hampered understanding of the spatio-temporal and subcellular distribution of RNA-guided processes. Here, we describe a universal protocol based on Arabidopsis to investigate subcellular RNA distribution from virtually any plant species using flow cytometry sorting. This protocol includes all necessary control steps to assess the quality of the nuclear RNA purification. Moreover, it can be easily applied to different plant developmental stages, tissues, cell cycle phases, experimental growth conditions, and specific cell type(s). For complete information on the use and execution of this protocol, please refer to and . The nature of plant tissues has continuously hampered understanding of the spatio-temporal and subcellular distribution of RNA-guided processes. Here, we describe a universal protocol based on Arabidopsis to investigate subcellular RNA distribution from virtually any plant species using flow cytometry sorting. This protocol includes all necessary control steps to assess the quality of the nuclear RNA purification. Moreover, it can be easily applied to different plant developmental stages, tissues, cell cycle phases, experimental growth conditions, and specific cell type(s).
Grants:	Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Ciencia e Innovación FJC2018-036091-I Ministerio de Ciencia e Innovación PRE2019-089555 Ministerio de Ciencia e Innovación PGC2018-101075-A-I00 Ministerio de Ciencia e Innovación CEX2019-000902-S
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cell Biology ; Flow Cytometry/Mass Cytometry ; Genomics ; RNA-seq ; Model Organisms
Published in:	STAR Protocols, Vol. 2, Issue 1 (March 2021) , art. 100320, ISSN 2666-1667

DOI: 10.1016/j.xpro.2021.100320
PMID: 33659901

19 p, 3.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles