Experimental comparison in sensing breast cancer mutations by signal on and signal off paper-based electroanalytical strips
Cinti, Stefano (Università degli Studi di Napoli Federico II)
Cinotti, Giulia (Università di Roma II "Tor Vergata". Dipartimento di Scienze e Tecnologie Chimiche)
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia)
Nguyen, Emily P. (Institut Català de Nanociència i Nanotecnologia)
Caratelli, Veronica (Università di Roma II "Tor Vergata". Dipartimento di Scienze e Tecnologie Chimiche)
Moscone, Danila (Università di Roma II "Tor Vergata". Dipartimento di Scienze e Tecnologie Chimiche)
Arduini, Fabiana (Università di Roma II "Tor Vergata". Dipartimento di Scienze e Tecnologie Chimiche)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Data:	2020
Resum:	The development of paper-based electroanalytical strips as powerful diagnostic tools has gained a lot of attention within the sensor community. In particular, the detection of nucleic acids in complex matrices represents a trending topic, especially when focused toward the development of emerging technologies, such as liquid biopsy. DNA-based biosensors have been largely applied in this direction, and currently, there are two main approaches based on target/probe hybridization reported in the literature, namely Signal ON and Signal OFF. In this technical note, the two approaches are evaluated in combination with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense mutation in exon 20 in breast cancer as the model target. A detailed comparison among the analytical performances, detection protocol, and cost associated with the two systems is provided, highlighting the advantages and drawbacks depending on the application. The present work is aimed to a wide audience, particularly for those in the field of point-of-care, and it is intended to provide the know-how to manage with the design and development stages, and to optimize the platform for the sensing of nucleic acids using a paper-based detection method.
Ajuts:	European Commission 795635 European Commission 794007 Agencia Estatal de Investigación MAT2017-87202-P Ministerio de Economía y Competitividad SEV-2017-0706
Nota:	Altres ajuts: the ICN2 is funded by the CERCA Programme/Generalitat de Catalunya.
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Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Analytical performance ; Design and Development ; Detection methods ; Detection protocols ; DNA-based biosensors ; Emerging technologies ; Experimental comparison ; Single strand DNA
Publicat a:	Analytical chemistry, Vol. 92, issue 2 (Jan. 2020) , p. 1674-1679, ISSN 1520-6882

DOI: 10.1021/acs.analchem.9b02560

Postprint 8 p, 360.6 KB

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