Genome Sequence, Proteome Profile, and Identification of a Multiprotein Reductive Dehalogenase Complex in Dehalogenimonas alkenigignens Strain BRE15M
Trueba-Santiso, Alba (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Wasmund, Kenneth (University of Vienna)
Soder-Walz, Jesica M (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Marco Urrea, Ernest (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Adrian, Lorenz (Helmholtz Centre for Environmental Research)

Data:	2020
Resum:	Bacteria of the genus Dehalogenimonas respire with vicinally halogenated alkanes via dihaloelimination. We aimed to describe involved proteins and their supermolecular organization. Metagenomic sequencing of a Dehalogenimonas -containing culture resulted in a 1. 65 Mbp draft genome of Dehalogenimonas alkenigignens strain BRE15M. It contained 31 full-length reductive dehalogenase homologous genes (rdhA), but only eight had cognate rdhB gene coding for membrane-anchoring proteins. Shotgun proteomics of cells grown with 1,2-dichloropropane as an electron acceptor identified 1152 proteins representing more than 60% of the total proteome. Ten RdhA proteins were detected, including a DcpA ortholog, which was the strongest expressed RdhA. Blue native gel electrophoresis (BNE) demonstrating maximum activity was localized in a protein complex of 146-242 kDa. Protein mass spectrometry revealed the presence of DcpA, its membrane-anchoring protein DcpB, two hydrogen uptake hydrogenase subunits (HupL and HupS), an iron-sulfur protein (HupX), and subunits of a redox protein with a molybdopterin-binding motif (OmeA and OmeB) in the complex. BNE after protein solubilization with different detergent concentrations revealed no evidence for an interaction between the putative respiratory electron input module (HupLS) and the OmeA/OmeB/HupX module. All detected RdhAs comigrated with the organohalide respiration complex. Based on genomic and proteomic analysis, we propose quinone-independent respiration in Dehalogenimonas.
Ajuts:	Ministerio de Economía y Competitividad CTM2016-75587-C2-1-R Agencia Estatal de Investigación PID2019-103989RB-I00 Ministerio de Economía y Competitividad BES-2014-070817 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-14
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Organohalide respiration ; Dihaloelimination ; Dehalogenimonas alkenigignens strain BRE15M ; Dehalococcoidia ; Genome sequencing ; 1,2-dichloropropane ; Proteome profiling
Publicat a:	Journal of Proteome Research, Vol. 20, Num. 1 (January 2021) , p. 613-623, ISSN 1535-3907

DOI: 10.1021/acs.jproteome.0c00569
PMID: 32975419

11 p, 2.3 MB

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