Lipid monolayer formation and lipid exchange monitored by a graphene field-effect transistor
Blaschke, Benno M. (Technische Universität München. Walter Schottky Institut)
Böhm, Philip (Ludwig-Maximilians-Universität. Center for NanoScience)
Drieschner, Simon ![Identificador ORCID](/img/uab/orcid.ico)
(Technische Universität München. Walter Schottky Institut)
Nickel, Bert ![Identificador ORCID](/img/uab/orcid.ico)
(Ludwig-Maximilians-Universität. Center for NanoScience)
Garrido, Jose ![Identificador ORCID](/img/uab/orcid.ico)
(Institut Català de Nanociència i Nanotecnologia)
Fecha: |
2018 |
Resumen: |
Anionic and cationic lipids are key molecules involved in many cellular processes; their distribution in biomembranes is highly asymmetric, and their concentration is well-controlled. Graphene solution-gated field-effect transistors (SGFETs) exhibit high sensitivity toward the presence of surface charges. Here, we establish conditions that allow the observation of the formation of charged lipid layers on solution-gated field-effect transistors in real time. We quantify the electrostatic screening of electrolyte ions and derive a model that explains the influence of charged lipids on the ion sensitivity of graphene SGFETs. The electrostatic model is validated using structural information from X-ray reflectometry measurements, which show that the lipid monolayer forms on graphene. We demonstrate that SGFETs can be used to detect cationic lipids by self-exchange of lipids. Furthermore, SGFETs allow measuring the kinetics of layer formation induced by vesicle fusion or spreading from a reservoir. Because of the high transconductance and low noise of the electrical readout, we can observe characteristic conductance spikes that we attribute to bouncing-off events of lipid aggregates from the SGFET surface, suggesting a great potential of graphene SGFETs to measure the on-off kinetics of small aggregates interacting with supported layers. |
Ayudas: |
European Commission 696656 Ministerio de Economía y Competitividad SEV-2013-0295
|
Derechos: |
Tots els drets reservats. ![](/img/licenses/InC.ico) |
Lengua: |
Anglès |
Documento: |
Article ; recerca ; Versió sotmesa a revisió |
Materia: |
Cellular process ;
Electrostatic modeling ;
Electrostatic screening ;
Graphene field-effect transistors ;
High sensitivity ;
Ion sensitivities ;
Structural information ;
X-ray reflectometry ;
Anions ;
Electricity ;
Graphite ;
Ions ;
Lipids |
Publicado en: |
Langmuir, Vol. 34, issue 14 (April 2018) , p. 4224-4233, ISSN 1520-5827 |
DOI: 10.1021/acs.langmuir.8b00162
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Registro creado el 2021-04-26, última modificación el 2024-01-19