Microfluidic in vitro platform for (nano)safety and (nano)drug efficiency screening
Kohl, Yvonne (Fraunhofer Institute for Biomedical Engineering)
Biehl, Margit (Fraunhofer Institute for Biomedical Engineering)
Spring, Sarah (Fraunhofer Institute for Biomedical Engineering)
Hesler, Michelle (Fraunhofer Institute for Biomedical Engineering)
Ogourtsov, Vladimir (University College Cork. Tyndall National Institute)
Todorovic, Miomir (University College Cork. Tyndall National Institute)
Owen, Joshua (University of Leeds. Institute of Thermofluids)
Elje, Elisabeth (University of Oslo. Department of Molecular Medicine)
Kopecka, Kristina (Slovak Academy of Sciences. Biomedical Research Center)
Moriones, Oscar Hernando (Institut Català de Nanociència i Nanotecnologia)
Bastús, Neus G. (Institut Català de Nanociència i Nanotecnologia)
Šimon, Peter (Slovak University of Technology in Bratislava. Institute of Physical Chemistry and Chemical Physics)
Dubaj, Tibor (Institute of Physical Chemistry and Chemical Physicsof SUT)
Rundén-Pran, Elise (Norwegian Institute for Air Research. Department for Environmental Chemistry)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
William, Nicola (University of Leeds. School of Chemistry)
von Briesen, Hagen (Fraunhofer Institute for Biomedical Engineering)
Wagner, Sylvia (Fraunhofer Institute for Biomedical Engineering)
Kapur, Nikil (University of Leeds. Institute of Thermofluids)
Mariussen, Espen (Norwegian Institute for Air Research. Department for Environmental Chemistry)
Nelson, Andrew (University of Leeds. School of Chemistry)
Gabelova, Alena (Slovak Academy of Sciences. Biomedical Research Center)
Dusinska, Maria (Norwegian Institute for Air Research. Department for Environmental Chemistry)
Velten, Thomas (Fraunhofer Institute for Biomedical Engineering)
Knoll, Thorsten (Fraunhofer Institute for Biomedical Engineering)

Date:	2021
Abstract:	Microfluidic technology is a valuable tool for realizing more in vitro models capturing cellular and organ level responses for rapid and animal-free risk assessment of new chemicals and drugs. Microfluidic cell-based devices allow high-throughput screening and flexible automation while lowering costs and reagent consumption due to their miniaturization. There is a growing need for faster and animal-free approaches for drug development and safety assessment of chemicals (Registration, Evaluation, Authorisation and Restriction of Chemical Substances, REACH). The work presented describes a microfluidic platform for in vivo-like in vitro cell cultivation. It is equipped with a wafer-based silicon chip including integrated electrodes and a microcavity. A proof-of-concept using different relevant cell models shows its suitability for label-free assessment of cytotoxic effects. A miniaturized microscope within each module monitors cell morphology and proliferation. Electrodes integrated in the microfluidic channels allow the noninvasive monitoring of barrier integrity followed by a label-free assessment of cytotoxic effects. Each microfluidic cell cultivation module can be operated individually or be interconnected in a flexible way. The interconnection of the different modules aims at simulation of the whole-body exposure and response and can contribute to the replacement of animal testing in risk assessment studies in compliance with the 3Rs to replace, reduce, and refine animal experiments.
Grants:	European Commission 857381 European Commission 685817 Ministerio de Economía y Competitividad SEV-2013-0295-17-3
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Drug efficiency ; In vitro culture-on-chip ; Microfluidic platform ; Miniaturized incubator microscope ; (nano)safety
Published in:	Small, Vol. 17, issue 15 (April 2021) , art. 2006012, ISSN 1613-6829

DOI: 10.1002/smll.202006012

15 p, 4.6 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles