Neuroprotective fragment C of tetanus toxin modulates IL-6 in an ALS mouse model
Moreno-Martinez, L. (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
De La Torre, M. (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
Muñoz, M. J. (Universidad de Zaragoza. Centro de Investigación Biomédica en Red Sobre Enfermedades)
Zaragoza, Pilar (Universidad de Zaragoza. Centro de Investigación Biomédica en Red Sobre Enfermedades)
Aguilera, José (Universitat Autònoma de Barcelona. Institut de Neurociències)
Calvo, Ana Cristina (Universidad de Zaragoza. Centro de Investigación Biomédica en Red Sobre Enfermedades)
Osta, R. (Universidad de Zaragoza. Centro de Investigación Biomédica en Red Sobre Enfermedades)

Date:	2020
Abstract:	Neuroinflammation plays a significant role in amyotrophic lateral sclerosis (ALS) pathology, leading to the development of therapies targeting inflammation in recent years. Our group has studied the tetanus toxin C-terminal fragment (TTC) as a therapeutic molecule, showing neuroprotective properties in the SOD1G93A mouse model. However, it is unknown whether TTC could have some effect on inflammation. The objective of this study was to assess the effect of TTC on the regulation of inflammatory mediators to elucidate its potential role in modulating inflammation occurring in ALS. After TTC treatment in SOD1G93A mice, levels of eotaxin-1, interleukin (IL)-2, IL-6 and macrophage inflammatory protein (MIP)-1 alpha (ff) and galectin-1 were analyzed by immunoassays in plasma samples, whilst protein expression of caspase-1, IL-1β, IL-6 and NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) was measured in the spinal cord, extensor digitorum longus (EDL) muscle and soleus (SOL) muscle. The results showed reduced levels of IL-6 in spinal cord, EDL and SOL in treated SOD1G93A mice. In addition, TTC showed a different role in the modulation of NLRP3 and caspase-1 depending on the tissue analyzed. In conclusion, our results suggest that TTC could have a potential anti-inflammatory effect by reducing IL-6 levels in tissues drastically affected by the disease. However, further research is needed to study more in depth the anti-inflammatory effect of TTC in ALS.
Grants:	Instituto de Salud Carlos III PI17/00949
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	TTC ; Amyotrophic lateral sclerosis ; Inflammation ; SOD1G93A mouse model
Published in:	Toxins, Vol. 12 Núm. 5 (may 2020) , p. 330, ISSN 2072-6651

Adreça alternativa: https://www.mdpi.com/2072-6651/12/5/330
DOI: 10.3390/toxins12050330
PMID: 32429516

10 p, 1.2 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
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Articles > Published articles