Phylogenetic relationships investigation of Mycobacterium caprae strains from sympatric wild boar and goats based on whole genome sequencing
Ciaravino, Giovanna (Universitat Autònoma de Barcelona)
Vidal Barba, Enric (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Cortey, Martí (Universitat Autònoma de Barcelona)
Martín, Maite (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Sanz, Albert (Generalitat de Catalunya. Departament d'Agricultura, Ramaderia, Pesca i Alimentació (DARP))
Mercader, Irene (Generalitat de Catalunya. Departament d'Agricultura, Ramaderia, Pesca i Alimentació (DARP))
Perea, Claudia (United States Department of Agriculture)
Robbe-Austerman, Suelee (United States Department of Agriculture)
Allepuz Palau, Alberto (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Pérez de Val, Bernat (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Date:	2020
Abstract:	Tuberculosis (TB) in wildlife challenges epidemiological surveillance and disease control. An outbreak of TB was detected in a free-ranging wild boar population of a Natural Park in Catalonia (Spain) and the outbreak investigation was conducted in the area. During the study period (2015-2020), 278 wild boars were analysed by gross pathology, histopathology, mycobacterial culture and DVR-spoligotyping. In addition, all cattle (49) and goat (47) herds of the area were tested with tuberculin skin test. TB compatible lesions were detected in 21 wild boars, and Mycobacterium caprae was isolated in 17 of them with two different spoligotypes: SB0415 (13) and SB1908 (4). Only two goat herds showed TB positive animals that were subsequently slaughtered. M. caprae with the spoligotypes SB0416 and SB0415 were isolated from these animals. To investigate the phylogenetic relationships and the transmission chain of the outbreak, nine strains isolated from six wild boars and three goats of the study area were analysed by whole genome sequencing (WGS) followed by single nucleotide polymorphism (SNP) analysis by maximum likelihood and median-joining network inference methods. Results indicated that infected wild boars maintained M. caprae strains circulation in their own population and have likely transmitted the infection to goats, thus acting as TB reservoirs, compromising the success of livestock TB eradication campaigns and posing a risk for public health. The results also highlighted the usefulness of WGS followed by SNP analysis in providing relevant epidemiological information when detailed contact data are missing.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Epidemiology ; Goats ; Mycobacterium caprae ; Tuberculosis ; Whole genome sequencing ; Wild boar
Published in:	Transboundary and Emerging Diseases, Vol. 68 (september 2020) , p. 1476-1486, ISSN 1865-1682

DOI: 10.1111/tbed.13816
PMID: 32888386

11 p, 2.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > GIES (Grup de Recerca en Estrès i Salut)
Articles > Research articles
Articles > Published articles