A rapid test for the environmental detection of pigeon antigen
Sánchez Díez, Silvia (Hospital Universitari Vall d'Hebron)
Cruz Carmona, María Jesús (Hospital Universitari Vall d'Hebron)
Álvarez-Simón, Daniel (Hospital Universitari Vall d'Hebron)
Montalvo Porro, Tomas (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Muñoz Gall, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Hoet, Peter M. (Department of Public Health and Primary Care, Centre for Environment and Health, Leuven)
Vanoirbeek, Jeroen A. (Department of Public Health and Primary Care, Centre for Environment and Health, Leuven)
Gómez-Ollés, Susana (Hospital Universitari Vall d'Hebron)

Data:	2021
Resum:	Avoidance of inhaled bird antigens is essential to prevent hypersensitivity pneumonitis disease progression. The aim of the present study was to develop a sandwich enzyme link immunoassay (ELISA) and an immunochromatographic test (ICT) and compare their ability to detect pigeon antigens in environmental samples. An amplified sandwich ELISA using pigeon serum as a calibration standard and a ICT using gold-labeled anti-pigeon serum antibodies for the rapid detection of pigeon antigens in environmental samples were developed. Twenty-two different airborne samples were collected and analysed using both methods. Strip density values obtained with ICT were calculated and compared with the concentrations determined by the ELISA method for pigeon antigens. Strips results were also visually analysed by five independent evaluators. The ELISA method to quantify pigeon antigen had a broader range (58. 4 and 10,112. 2 ng/ml), compared to the ICT assay (420 to 3360 ng/ml). A kappa index of 0. 736 (p < 0. 0001) was obtained between the observers evaluating the ICT strips. The results of the ELISA and the relative density of the ICT showed a highly significant correlation (rs:0. 935; p < 0. 0001). Bland-Altman plot also confirmed excellent agreement between the two methods (mean difference: -1. 626; p < 0. 0001). Since there was a good correlation between both assays, we can conclude that the rapid and simple ICT assay is a good and valid alternative, which does not require expensive equipment, for the validated ELISA technique.
Ajuts:	Instituto de Salud Carlos III MSII17/00025 Ministerio de Economía y Competitividad PI15/01954 European Commission 874707
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Hypersensitivity pneumonitis ; Immunochromatographic test ; Pigeon antigen ; Strip assay
Publicat a:	Science of the total environment, Vol. 788 (september 2021) , ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2021.147789
PMID: 34134383

8 p, 1.2 MB

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