Home > Articles > Published articles > Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology : |
Date: | 2022 |
Abstract: | Serological tests are essential for the control and management of COVID-19 pandemic (diagnostics and surveillance, and epidemiological and immunity studies). We introduce a direct serological biosensor assay employing proprietary technology based on plasmonics, which offers rapid (<15 min) identification and quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies in clinical samples, without signal amplification. The portable plasmonic device employs a custom-designed multiantigen (RBD peptide and N protein) sensor biochip and reaches detection limits in the low ng mL -1 range employing polyclonal antibodies. It has also been implemented employing the WHO-approved anti-SARS-CoV-2 immunoglobulin standard. A clinical validation with COVID-19 positive and negative samples (n = 120) demonstrates its excellent diagnostic sensitivity (99%) and specificity (100%). This positions our biosensor as an accurate and easy-to-use diagnostics tool for rapid and reliable COVID-19 serology to be employed both at laboratory and decentralized settings for the disease management and for the evaluation of immunological status during vaccination or treatment. |
Grants: | European Commission 101003544 Ministerio de Ciencia e Innovación SEV-2017-0706 Ministerio de Economía, Industria y Competitividad BES-2017-080527 Ministerio de Economía y Competitividad TEC 2016-78515-R European Commission 871029 European Commission 644956 Ministerio de Economía y Competitividad RD16/0016/0003 |
Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Language: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Subject: | Immunology ; Sensors ; Peptides and proteins ; Assays ; Biopolymers |
Published in: | Analytical chemistry, Vol. 94, Issue 2 (January 2022) , p. 975-984, ISSN 1520-6882 |
10 p, 3.1 MB |