Mycobacterium tuberculosis Infection Up-Regulates Sialyl Lewis X Expression in the Lung Epithelium
Matos, Rita (ICBAS-Instituto de Ciências Biomédicas Abel Salazar, University of Porto)
Fonseca, Kaori L. (Programa de Pós-Graduação Ciência para o Desenvolvimento (PGCD), Instituto Gulbenkian de Ciência (IGC))
Mereiter, Stefan (IPATIMUP-Instituto de Patologia e Imunologia Molecular da Universidade do Porto)
Maceiras, Ana Raquel (IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto)
Gomes, Joana (IPATIMUP-Instituto de Patologia e Imunologia Molecular da Universidade do Porto)
Vilaplana, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Gartner, Fátima (ICBAS-Instituto de Ciências Biomédicas Abel Salazar, University of Porto)
Rodrigues, Pedro N. S. (IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto)
Reis, Celso A. (FMUP-Faculdade de Medicina da Universidade do Porto)
Saraiva, Margarida (IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto)
Magalhães, Ana (IPATIMUP-Instituto de Patologia e Imunologia Molecular da Universidade do Porto)

Date:	2021
Abstract:	Glycans display increasingly recognized roles in pathological contexts, however, their impact in the host-pathogen interplay in many infectious diseases remains largely unknown. This is the case for tuberculosis (TB), one of the ten most fatal diseases worldwide, caused by infection of the bacteria Mycobacterium tuberculosis. We have recently reported that perturbing the core-2 O -glycans biosynthetic pathway increases the host susceptibility to M. tuberculosis infection, by disrupting the neutrophil homeostasis and enhancing lung pathology. In the present study, we show an increased expression of the sialylated glycan structure Sialyl-Lewis X (SLeX) in the lung epithelium upon M. tuberculosis infection. This increase in SLeX glycan epitope is accompanied by an altered lung tissue transcriptomic signature, with up-regulation of genes codifying enzymes that are involved in the SLeX core-2 O -glycans biosynthetic pathway. This study provides novel insights into previously unappreciated molecular mechanisms involving glycosylation, which modulate the host response to M. tuberculosis infection, possibly contributing to shape TB disease outcome.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Mycobacterium tuberculosis ; Lung glycophenotype ; Lewis antigens ; Sialyl-Lewis X
Published in:	Microorganisms, Vol. 9 (january 2021) , ISSN 2076-2607

DOI: 10.3390/microorganisms9010099
PMID: 33406734

14 p, 2.6 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles