Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
Royo, Felix (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Azkargorta, Mikel (Center for Cooperative Research in Biosciences, Bizkaia Technology Park)
Lavin, Jose L. (Center for Cooperative Research in Biosciences, Bizkaia Technology Park)
Clos-Garcia, Marc (Center for Cooperative Research in Biosciences, Bizkaia Technology Park)
Cortazar, Ana Rosa (Centro de Investigación Biomédica en Red de Cáncer)
Gonzalez-Lopez, Monika (Center for Cooperative Research in Biosciences, Bizkaia Technology Park)
Barcena, Laura (Center for Cooperative Research in Biosciences, Bizkaia Technology Park)
Del Portillo, Hernando A.. (Institució Catalana de Recerca i Estudis Avançats)
Yáñez-Mó, María (Instituto de Investigación Hospital Universitario de la Princesa)
Marcilla, Antonio (Instituto de Investigación Sanitaria La Fe)
Borràs i Serres, Francesc Enric (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Peinado, Héctor (Microenvironment and Metastasis Laboratory, Molecular Oncology Program, Spanish National Cancer Research Center)
Guerrero, Isabel (Centro de Biología Molecular Severo Ochoa)
Váles-Gómez, Mar (Spanish National Centre for Biotechnology, Spanish National Research Council)
Cereijo, Unai (Anaxomics Biotech, Barcelona)
Sardon, Teresa (Anaxomics Biotech, Barcelona)
Aransay, Ana M. (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Elortza, Felix (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Falcón-Pérez, Juan M (Ikerbasque, Basque Foundation for Science)

Date:	2021
Abstract:	Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.
Grants:	Ministerio de Economía y Competitividad SAF2015-71231-REDT Ministerio de Ciencia e Innovación RTI2018-094969-B-I00
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Extracellular vesicles (EVs) ; Exosomes ; MLP29 ; Fibroblast ; Cell crosstalk ; Immune response
Published in:	Frontiers in Cell and Developmental Biology, Vol. 8 (january 2021) , ISSN 2296-634X

DOI: 10.3389/fcell.2020.613583
PMID: 33511119

10 p, 1.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles