Genotyping Porcine Circovirus 3 (PCV-3) Nowadays : Does It Make Sense?
Franzo, Giovanni (Padua University. Department of Animal Medicine, Production and Health)
Delwart, Eric (University of California. Department of Laboratory Medicine)
Fux, Robert (Institute for Infectious Diseases and Zoonoses. Department for Veterinary Sciences)
Hause, Ben (Kansas State University. Kansas State Veterinary Diagnostic Laboratory)
Su, Shuo (Nanjing Agricultural University. Joint International Research Laboratory of Animal Health and Food Safety)
Zhou, JiYong (Zhejiang University. Key Laboratory of Animal Virology and Department of Veterinary Medicine)
Segalés Coma, Joaquim (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Data:	2020
Resum:	The discovery of a globally distributed porcine circovirus (Porcine circovirus 3 ; PCV-3) has led to intense research activity and the production of a large amount of molecular data. Different research groups have proposed several, not always concordant, genotypes for this virus. While such categories could aid an easier interpretation of PCV-3 molecular epidemiology, any classification, to be useful in practical settings, must be univocal and of help in the understanding of underlying biological features and epidemiology. Based on these premises, the possibility of defining PCV-3 genotypes was evaluated on the broadest available dataset of PCV-3 complete genome (n = 357) and open reading frame 2 (ORF2, n = 653) sequences. Genetic distance and phylogenetic clustering were selected as the main objective criteria. Additional factors, including the number of within-cluster sequences, host and geographic clustering, concordance between different genomic regions, and analysis method were also taken in account to generate a classification that could be effectively applied in research and diagnostic settings. A maximum within-genotype genetic distance of 3% at the complete genome and 6% at the ORF2 levels, bootstrap support higher than 90%, and concordance between analysis methods allowed us to clearly define two clades which could be potentially defined as genotypes. Further subdivision was not suggested due to the absence of a meaningful association between PCV-3 and its biological/epidemiological features. Nevertheless, since one of the clades included two strains only, thus far we formally propose the definition of only one PCV-3 genotype (PCV-3a). The established criteria will allow us to automatically recognize other genotypes when more strain sequences are characterized.
Nota:	Altres ajuts: Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria E-RTA2017-00007-00-00 INIA
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	PCV-3 ; Genotypes ; Classification ; ORF2 ; Genome
Publicat a:	Viruses, Vol. 12 (february 2020) , ISSN 1999-4915

DOI: 10.3390/v12030265
PMID: 32121102

13 p, 1.6 MB

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