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Pàgina inicial > Articles > Articles publicats > Unravelling the consequences of the bacteriophages in human samples |
Data: | 2020 |
Resum: | Bacteriophages are abundant in human biomes and therefore in human clinical samples. Although this is usually not considered, they might interfere with the recovery of bacterial pathogens at two levels: 1) by propagating in the enrichment cultures used to isolate the infectious agent, causing the lysis of the bacterial host and 2) by the detection of bacterial genes inside the phage capsids that mislead the presence of the bacterial pathogen. To unravel these interferences, human samples (n = 271) were analyzed and infectious phages were observed in 11% of blood culture, 28% of serum, 45% of ascitic fluid, 14% of cerebrospinal fluid and 23% of urine samples. The genetic content of phage particles from a pool of urine and ascitic fluid samples corresponded to bacteriophages infecting different bacterial genera. In addition, many bacterial genes packaged in the phage capsids, including antibiotic resistance genes and 16S rRNA genes, were detected in the viromes. Phage interference can be minimized applying a simple procedure that reduced the content of phages up to 3 logs while maintaining the bacterial load. This method reduced the detection of phage genes avoiding the interference with molecular detection of bacteria and reduced the phage propagation in the cultures, enhancing the recovery of bacteria up to 6 logs. |
Ajuts: | Instituto de Salud Carlos III PI16/00158 Instituto de Salud Carlos III AGL2016-75536-P Instituto de Salud Carlos III CD15/00017 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-170 Ministerio de Economía, Industria y Competitividad BES-2017-081296 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Altres ; recerca ; Versió publicada |
Matèria: | Bacteriophages ; Bacterial infection |
Publicat a: | Scientific reports, Vol. 10 (april 2020) , ISSN 2045-2322 |
10 p, 1.8 MB |