RNA-Seq analysis of European sea bass (Dicentrarchus labrax L.) infected with nodavirus reveals powerful modulation of the stress response
Lama, Raquel (Instituto de Investigaciones Marinas (Vigo, Espanya))
Pereiro, Patricia (Instituto de Investigaciones Marinas (Vigo, Espanya))
Valenzuela-Muñoz, Valentina (Centro Interdisciplinario para la Investigación Acuícola (Xile))
Gallardo-Escárate, Cristian (Centro Interdisciplinario para la Investigación Acuícola (Xile))
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Figueras, Antonio (Instituto de Investigaciones Marinas (Vigo, Espanya))
Novoa, Beatriz (Instituto de Investigaciones Marinas (Vigo, Espanya))

Data:	2020
Resum:	Nodavirus, or nervous necrosis virus (NNV), is the causative agent of viral encephalopathy and retinopathy (VER), a severe disease affecting numerous fish species worldwide. European sea bass, a cultured species of great economic importance, is highly susceptible to the disease. To better understand the response of this organism to NNV, we conducted RNA-Seq analysis of the brain and head kidney from experimentally infected and uninfected sea bass juveniles at 24 and 72 hours post-infection (hpi). Contrary to what was expected, we observed modest modulation of immune-related genes in the brain, the target organ of this virus, and some of these genes were even downregulated. However, genes involved in the stress response showed extremely high modulation. Accordingly, the genes encoding the enzymes implicated in the synthesis of cortisol were almost the only overexpressed genes in the head kidney at 24 hpi. This stress response was attenuated after 72 h in both tissues, and a progressive immune response against the virus was mounted. Moreover, experiments were conducted to determine how stress activation could impact NNV replication. Our results show the complex interplay between viral activity, the stress reaction and the immune response.
Ajuts:	European Commission 727610 Agencia Estatal de Investigación BIO2017-82851-C3-2-R
Nota:	Altres ajuts: Raquel Lama and Patricia Pereiro wish to thank the Axencia Galega de Innovación (GAIN, Xunta de Galicia) for their predoctoral (IN606A-2017/011) and postdoctoral (IN606B-2018/010) contracts, respectively. Our laboratory is funded by EU Feder Programa Interreg España-Portugal OPE01833 and IN607B 2019/01 from the Consellería de Economía, Emprego e Industria (GAIN), Xunta de Galicia.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Veterinary research, Vol. 51 (2020) , art. 64, ISSN 1297-9716

DOI: 10.1186/s13567-020-00784-y
PMID: 32398117

22 p, 3.2 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats