Immunohistochemical Studies of Cytokeratins and Differentiation Markers in Bovine Ocular Squamous Cell Carcinoma
Vala, Helena (University of Trás-os-Montes and Alto Douro. Centre for the Research and Technology of Agro-Environmental and Biological Sciences)
Carvalho, Tânia (Universidade de Lisboa. Instituto de Medicina Molecular)
Pinto, Carlos (Universidade dos Açores. Faculdade de Ciências Agrárias e do Ambiente)
Pereira, Maria A. (Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical)
Mesquita, João R. (Universidade do Porto. Instituto de Ciências Biomédicas Abel Salazar)
Peleteiro, Maria C. (Universidade de Lisboa. Faculdade de Medicina Veterinária)
Ferrer i Caubet, Lluís (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Fondevila, Dolors (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2020
Resum:	Bovine Ocular Squamous Cell Carcinoma is considered the most common bovine tumour, causing significant economic losses, mainly by abattoir condemnations. To obtain a better insight into the genesis and neoplastic transformation, 19 samples collected at slaughter from Holstein Friesian cattle and diagnosed as Ocular Squamous Cell Carcinoma were studied. Tumours were histologically classified into three categories: poorly (26. 3%), moderately (26. 3%), and well differentiated (47. 4%). Expression of keratins (MNF116 and LP34) and of cornified envelope precursors (involucrin and profilaggrin) was studied. Expression of MNF116 was observed in all carcinomas. LP34 immunostaining was seen in all but three carcinomas, one from each degree. Involucrin immunoreaction was observed in all but one poorly differentiated carcinoma. Profilaggrin was present in only two moderately differentiated carcinomas, in all but one well differentiated, and in all but one poorly differentiated. MNF116 is a useful marker to confirm the epithelial origin of the tumour and stain most neoplastic cells in these tumours. The expression of involucrin and LP34 demonstrates that, in all tumours, cells have reached the final program of differentiation, regardless of the grade. The expression of profilaggrin could indicate molecular changes during malignant transformation but their expression does not seem to be of diagnostic value.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Bovine ; Cytokeratin ; Immunohistochemistry ; Involucrin ; Ocular squamous cell carcinoma ; Profilaggrin
Publicat a:	Veterinary Sciences, Vol. 7 (may 2020) , ISSN 2306-7381

DOI: 10.3390/vetsci7020070
PMID: 32485997

14 p, 3.9 MB

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