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| Pàgina inicial > Articles > Articles publicats > Microbial Signature in Adipose Tissue of Crohn's Disease Patients |
(Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas) (Hospital Universitario Virgen de la Victoria (Màlaga, Andalusia)) (Hospital Universitari Joan XXIII de Tarragona) (Hospital Universitari Joan XXIII de Tarragona) (Hospital Universitario Virgen de la Victoria (Màlaga, Andalusia)) (Hospital Universitari Vall d'Hebron. Institut de Recerca) (Hospital Universitari Vall d'Hebron. Institut de Recerca) (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas) (Universitat Rovira i Virgili)| Data: | 2020 |
| Resum: | Crohn's disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity. |
| Ajuts: | Instituto de Salud Carlos III PI18/00037 Ministerio de Economía y Competitividad PI15/00143 Ministerio de Economía y Competitividad PI14/00228 Instituto de Salud Carlos III PI17/01503 Ministerio de Economía y Competitividad PI15/00256 Agencia Estatal de Investigación RTI2018-093919 Instituto de Salud Carlos III CB07708/0012 Ministerio de Economía y Competitividad RYC2013-13186 Ministerio de Economía y Competitividad CP10/00438 Instituto de Salud Carlos III CM18/00029 |
| Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Llengua: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Matèria: | Tissue microbiota ; Inflammatory bowel disease ; 16S sequencing ; Creeping fat ; PICRUSt analysis ; Escherichia coli ; Fusobacterium ; Lipopolysaccharide biosynthesis |
| Publicat a: | Journal of clinical medicine, Vol. 9 (july 2020) , ISSN 2077-0383 |
