Diagnostic benefits of adding EspC, EspF and Rv2348-B to the QuantiFERON Gold In-tube antigen combination
Villar-Hernández, Raquel (Universitat Autònoma de Barcelona)
Blauenfeldt, Thomas (Statens Serum Institut)
García-García, Esther (Universitat Autònoma de Barcelona)
Muriel-Moreno, Beatriz (Universitat Autònoma de Barcelona)
De Souza Galvão, Maria Luiza (Hospital Universitari Vall d'Hebron)
Millet, Joan-Pau (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Sabriá, Fina (Hospital de Sant Joan Despí Moisès Broggi)
Sánchez-Montalvá, Adrian (Hospital Universitari Vall d'Hebron)
Ruiz-Manzano, Juan (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pilarte, Jose (Hospital Universitari Vall d'Hebron)
Jiménez, M. A. (Hospital Universitari Vall d'Hebron)
Centeno Clemente, Carmen Angela (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Martos, Carmen (Hospital de Sant Joan Despí Moisès Broggi)
Molina-Pinargote, Israel (Unitat Clínica de Tractament Directament Observat de la Tuberculosi (Barcelona))
González-Díaz, Yoel D. (Unitat Clínica de Tractament Directament Observat de la Tuberculosi (Barcelona))
Santiago, Javier (Unitat Clínica de Tractament Directament Observat de la Tuberculosi (Barcelona))
Cantos, Adela (Hospital Universitari Vall d'Hebron)
Casas, Irma (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Guerola, Rosa (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Prat Aymerich, Cristina (Utrecht University)
Andersen, Peter Lawætz (Statens Serum Institut)
Latorre, Irene (Universitat Autònoma de Barcelona)
Ruhwald, Morten (Statens Serum Institut)
Domínguez, Jose (Universitat Autònoma de Barcelona)

Date:	2020
Abstract:	Interferon (IFN)-γ release assays (IGRAs) are used to diagnose latent tuberculosis (TB) infection (LTBI). To improve the accuracy of these tests, different approaches, such as alternative cytokine detection and using different antigens, are considered. Following this purpose, this study aims to evaluate the addition of EspC, EspF and Rv2348-B to those present in the QuantiFERON-TB Gold In-Tube (QFN-G-IT). We included 115 subjects: 74 active TB patients, 17 LTBI individuals and 24 healthy controls. Whole blood samples were collected in QFN-G-IT and in-house tubes containing different combinations of EspC, EspF and Rv2348-B, together with ESAT-6, CFP-10, and TB7. 7. After overnight incubation at 37 ºC, plasma was harvested and IFN-γ quantified. IFN-γ levels in the QFN-G-IT and in-house tubes correlated very good (Spearman Rho(r) > 0. 86). In-house antigen combinations distinguished healthy individuals from those with active TB and LTBI (specificities and sensitivities higher than 87. 5% and 96. 3%, respectively [AUC > 0. 938]). Adding EspC, EspF and Rv2348-B, increased the sensitivity of the test, being the addition of EspC and Rv2348-B the combination that yielded a higher sensitivity with no specificity loss. Addition of these antigens could improve diagnosis in patients with impaired or immature immune response who are at high risk of developing TB.
Grants:	Instituto de Salud Carlos III PI16/01912 Instituto de Salud Carlos III PI18/00411 Ministerio de Economía, Industria y Competitividad JE18/0002
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Diagnostic markers ; Tuberculosis
Published in:	Scientific reports, Vol. 10 (august 2020) , ISSN 2045-2322

DOI: 10.1038/s41598-020-70204-w
PMID: 32764560

11 p, 1.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles