Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis
Molina-Moya, Barbara (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Ciobanu, Nelly (Institutia Medico-Sanitara Publica)
Hernandez, Marta (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Prat i Aymerich, Cristina (Institut Germans Trias i Pujol)
Crudu, Valeriu (Institutia Medico-Sanitara Publica)
Adams, Emily (Liverpool School of Tropical Medicine. Centre for Drugs and Diagnostics)
Codreanu, Alexandru (Institutia Medico-Sanitara Publica)
Sloan, Derek J. (University of St Andrews. School of Medicine)
Cuevas, Luis E.. (Liverpool School of Tropical Medicine. Department of Clinical Sciences)
Domínguez, José (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2020
Resum:	Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are needed. We evaluated whether TB can be detected from the oral mucosa of patients with TB. Adults with presumptive TB were examined using culture, Xpert MTB/RIF, smear microscopy and X-Rays. Oral mucosa swabs collected on PrimeStore-MTM, stored at room temperature if tested within 30 days or at −20 °C if examined at a later time. RT-PCR was performed to detect M. tuberculosis DNA. Eighty patients had bacteriologically-confirmed TB, 34 had bacteriologically-negative TB (negative tests but abnormal X-rays) and 152 were considered not to have TB (not TB). Oral swabs RT-PCR were positive in 29/80 (36. 3%) bacteriologically-confirmed, 9/34 (26. 5%) bacteriologically-negative and 29/152 (19. 1%) not TB. The yield varied among samples stored for less and more than 30 days (p = 0. 013) from 61% (11/18) and 29% (18/62) among bacteriologically confirmed, and 30. 8% (4/13) and 23. 8% (5/21) among bacteriologically-negative participants. Among not TB patients, the specificity was 80. 9% (123/152), being 78. 3% (18/23) among samples stored less than 30 days and 81. 4% (105/129) among samples stored for more than 30 days (p = 0. 46). The detection of M. tuberculosis in oral mucosa samples is feasible, but storage conditions may affect the yield.
Ajuts:	Instituto de Salud Carlos III PI116-01912 European Commission 823854
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Oral swab ; Diagnosis ; Real-time PCR
Publicat a:	Journal of clinical medicine, Vol. 9, Issue 12 (December 2020) , art. 4124, ISSN 2077-0383

DOI: 10.3390/jcm9124124
PMID: 33371314

7 p, 230.2 KB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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