Home > Articles > Published articles > Plasma microRNA Profiling Reveals Novel Biomarkers of Epicardial Adipose Tissue : |
Date: | 2019 |
Abstract: | Epicardial adipose tissue (EAT) constitutes a novel parameter for cardiometabolic risk assessment and a target for therapy. Here, we evaluated for the first time the plasma microRNA (miRNA) profile as a source of biomarkers for epicardial fat volume (EFV). miRNAs were profiled in plasma samples from 180 patients whose EFV was quantified using multidetector computed tomography. In the screening study, 54 deregulated miRNAs were identified in patients with high EFV levels (highest tertile) compared with matched patients with low EFV levels (lowest tertile). After filtering, 12 miRNAs were selected for subsequent validation. In the validation study, miR-15b-3p, miR-22-3p, miR-148a-3p miR-148b-3p and miR-590-5p were directly associated with EFV, even after adjustment for confounding factors (p value < 0. 05 for all models). The addition of miRNA combinations to a model based on clinical variables improved the discrimination (area under the receiver-operating-characteristic curve (AUC) from 0. 721 to 0. 787). miRNAs correctly reclassified a significant proportion of patients with an integrated discrimination improvement (IDI) index of 0. 101 and a net reclassification improvement (NRI) index of 0. 650. Decision tree models used miRNA combinations to improve their classification accuracy. These results were reproduced using two proposed clinical cutoffs for epicardial fat burden. Internal validation corroborated the robustness of the models. In conclusion, plasma miRNAs constitute novel biomarkers of epicardial fat burden. |
Grants: | Ministerio de Economía, Industria y Competitividad IJCI-2016-29393 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-1149 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-946 Instituto de Salud Carlos III PI14/01729 Instituto de Salud Carlos III PI16/00471 Instituto de Salud Carlos III PI18/01584 Instituto de Salud Carlos III CB16/11/00403 Instituto de Salud Carlos III CB16/11/00276 Instituto de Salud Carlos III CB07/08/0016 |
Note: | Altres ajuts: Project 201521-10 from Fundació MARATÓ TV3 (to V.L.-C.). O.B. (Project 201521-10) and N.P. (Project 201716) were recipients of Fundació Marató TV3. |
Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Language: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Subject: | Biomarker ; Cardiometabolic disease ; Epicardial adipose tissue ; Epicardial fat ; Epicardial fat volume ; Microrna |
Published in: | Journal of clinical medicine, Vol. 8 (june 2019) , ISSN 2077-0383 |
19 p, 2.1 MB |