A portable epigenetic switch for bistable gene expression in bacteria
Olivenza, David R. (Universidad de Sevilla. Departamento de Genética)
Nicoloff, Hervé (Uppsala University. Department of Medical Biochemistry and Microbiology)
Sánchez-Romero, María Antonia (Universidad de Sevilla. Departamento de Genética)
Cota, Ignacio (Centre de Recerca en Agrigenòmica)
Andersson, Dan I. (Uppsala University. Department of Medical Biochemistry and Microbiology)
Casadesús, Josep (Universidad de Sevilla. Departamento de Genética)

Date:	2019
Abstract:	We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered o pvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.
Grants:	Ministerio de Ciencia e Innovación BIO2016-75235-P
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bacterial synthetic biology ; DNA methylation
Published in:	Scientific reports, Vol. 9 (August 2019) , art. 11261, ISSN 2045-2322

DOI: 10.1038/s41598-019-47650-2
PMID: 31375711

9 p, 1.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles