Serum microRNA-1 and microRNA-133a levels reflect myocardial steatosis in uncomplicated type 2 diabetes
Gonzalo Calvo, David de (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
van der Meer, Rutger W. (Leiden University Medical Center)
Rijzewijk, Luuk J. (Kantonsspital Baden AG, Baden, Switzerland)
Smit, Johannes W. A. (University Medical Center Nijmegen)
Revuelta-López, Elena (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Nasarre, Laura (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Escolà-Gil, Joan Carles (Institut d'Investigació Biomèdica Sant Pau)
Lamb, Hildo J. (Leiden University Medical Center)
Llorente-Cortés, Vicenta (Parc de Recerca Biomèdica de Barcelona (PRBB))
Universitat Autònoma de Barcelona

Date:	2017
Abstract:	Using in vitro, in vivo and patient-based approaches, we investigated the potential of circulating microRNAs (miRNAs) as surrogate biomarkers of myocardial steatosis, a hallmark of diabetic cardiomyopathy. We analysed the cardiomyocyte-enriched miRNA signature in serum from patients with well-controlled type 2 diabetes and with verified absence of structural heart disease or inducible ischemia, and control volunteers of the same age range and BMI (N = 86), in serum from a high-fat diet-fed murine model, and in exosomes from lipid-loaded HL-1 cardiomyocytes. Circulating miR-1 and miR-133a levels were robustly associated with myocardial steatosis in type 2 diabetes patients, independently of confounding factors in both linear and logistic regression analyses (P < 0. 050 for all models). Similar to myocardial steatosis, miR-133a levels were increased in type 2 diabetes patients as compared with healthy subjects (P < 0. 050). Circulating miR-1 and miR-133a levels were significantly elevated in high-fat diet-fed mice (P < 0. 050), which showed higher myocardial steatosis, as compared with control animals. miR-1 and miR-133a levels were higher in exosomes released from lipid-loaded HL-1 cardiomyocytes (P < 0. 050). Circulating miR-1 and miR-133a are independent predictors of myocardial steatosis. Our results highlight the value of circulating miRNAs as diagnostic tools for subclinical diabetic cardiomyopathy.
Grants:	Ministerio de Economía y Competitividad PI14/01729 Ministerio de Economía y Competitividad CB16/11/00403 Ministerio de Economía y Competitividad CD14/00109
Note:	Altres ajuts: Fundació Marató TV3 (201521 10).
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 7 (march 2017) , ISSN 2045-2322

DOI: 10.1038/s41598-017-00070-6
PMID: 28246388

14 p, 1.9 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles