Effects on pig immunophysiology, PBMC proteome and brain neurotransmitters caused by group mixing stress and human-animal relationship
Valent, Daniel (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Arroyo, Laura (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Peña, Raquel (Universitat Autònoma de Barcelona. Servei de Bioquímica Clínica Veterinària)
Yu, Kuai (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Carreras, Ricard (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Mainau, Eva (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Velarde, Antonio (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Servei de Bioquímica Clínica Veterinària)

Date:	2017
Abstract:	Peripheral blood mononuclear cells (PBMC) are an interesting sample for searching for biomarkers with proteomic techniques because they are easy to obtain and do not contain highly abundant, potentially masking proteins. Two groups of pigs (n = 56) were subjected to mixing under farm conditions and afterwards subjected to different management treatments: negative handling (NH) and positive handling (PH). Serum and PBMC samples were collected at the beginning of the experiment one week after mixing (t0) and after two months of different handling (t2). Brain areas were collected after slaughter and neurotransmitters quantified by HPLC. Hair cortisol and serum acute phase proteins decreased and serum glutathione peroxidase increased at t2, indicating a lower degree of stress at t2 after adaptation to the farm. Differential gel electrophoresis (DIGE) was applied to study the effects of time and treatment on the PBMC proteome. A total of 54 differentially expressed proteins were identified, which were involved in immune system modulation, cell adhesion and motility, gene expression, splicing and translation, protein degradation and folding, oxidative stress and metabolism. Thirty-seven protein spots were up-regulated at t2 versus t0 whereas 27 were down-regulated. Many of the identified proteins share the characteristic of being potentially up or down-regulated by cortisol, indicating that changes in protein abundance between t0 and t2 are, at least in part, consequence of lower stress upon adaptation to the farm conditions after group mixing. Only slight changes in brain neurotransmitters and PBMC oxidative stress markers were observed. In conclusion, the variation in hair cortisol and serum APPs as well as the careful analysis of the identified proteins indicate that changes in protein composition in PBMC throughout time is mainly due to a decrease in the stress status of the individuals, following accommodation to the farm and the new group.
Grants:	Ministerio de Economía y Competitividad AGL2011-30598-C03-02 Ministerio de Economía y Competitividad AGL2011-30598-C03-01 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014-SGR-4
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Home-animal, Relacions
Published in:	PloS one, Vol. 12 (may 2017) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0176928
PMID: 28475627

24 p, 7.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles