Follow-up monitoring in a cat with leishmaniosis and coinfections with Hepatozoon felis and ' Candidatus Mycoplasma haemominutum'
Attipa, Charalampos (Royal Veterinary College. Pathobiology and Population Sciences)
Neofytou, Kyriaki (Cyvets Veterinary Centre)
Yiapanis, Christos (Cyvets Veterinary Centre)
Martínez-Orellana, Pamela (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Baneth, Gad (Hebrew University of Jerusalem. Koret School of Veterinary Medicine)
Nachum-Biala, Yaarit (Hebrew University of Jerusalem. Koret School of Veterinary Medicine)
Brooks-Brownlie, Harriet (University of Bristol. Molecular Diagnostic Unit)
Solano Gallego, Laia (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Tasker, Séverine (University of Bristol. Molecular Diagnostic Unit)

Date:	2017
Abstract:	A 6-year-old female neutered domestic shorthair cat from Cyprus was presented with multiple ulcerated skin nodules. Cytology and histopathology of the lesions revealed granulomatous dermatitis with intracytoplasmic organisms, consistent with amastigotes of Leishmania species. Biochemistry identified a mild hyperproteinaemia. Blood extraction and PCR detected Leishmania species, Hepatozoon species and ' Candidatus Mycoplasma haemominutum' (CMhm) DNA. Subsequent sequencing identified Hepatozoon felis. Additionally, the rRNA internal transcribed spacer 1 locus of Leishmania infantum was partially sequenced and phylogeny showed it to cluster with species derived from dogs in Italy and Uzbekistan, and a human in France. Allopurinol treatment was administered for 6 months. Clinical signs resolved in the second month of treatment with no deterioration 8 months post-treatment cessation. Quantitative PCR and ELISA were used to monitor L infantum blood DNA and antibody levels. The cat had high L infantum DNA levels pretreatment that gradually declined during treatment but increased 8 months post-treatment cessation. Similarly, ELISA revealed high levels of antibodies pretreatment, which gradually declined during treatment and increased slightly 8 months post-treatment cessation. The cat remained PCR positive for CMhm and Hepatozoon species throughout the study. There was no clinical evidence of relapse 24 months post-treatment. To our knowledge, this is the first clinical report of a cat with leishmaniosis with H felis and CMhm coinfections. The high L infantum DNA levels post-treatment cessation might indicate that although the lesions had resolved, prolonged or an alternative treatment could have been considered.
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Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Journal of feline medicine and surgery open reports, Vol. 3 (november 2017) , ISSN 2055-1169

DOI: 10.1177/2055116917740454
PMID: 29163980

6 p, 755.2 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Research articles
Articles > Published articles