Validation of an automated assay for the measurement of cupric reducing antioxidant capacity in serum of dogs
Rubio, Camila Peres (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Tvarijonaviciute, Asta (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Martínez-Subiela, Silvia (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Hernández-Ruiz, Josefa (Universidad de Murcia. Departamento de Biología Vegetal)
Cerón, José Joaquín (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)

Data:	2016
Resum:	The objective of the present study was to optimize and validate an automated method to assess the total antioxidant capacity (TAC) in serum of dogs using the cupric reducing antioxidant capacity (CUPRAC) methodology (TAC) with bathocuproinedisulfonic acid disodium salt as chelating agent, evaluating also possible variations due to the use of two different automated analyzers. The method is based on the reduction of Cu 2+ into Cu 1+ by the action of the non-enzymatic antioxidants that are present in the sample. Imprecision was low in both apparatus utilized, and the results were linear across serial Trolox and canine serum samples dilutions. Lipids did not interfere with the assay; however, hemolysis increased the TAC concentrations. When TAC concentrations were determined in ten healthy (control) dogs and in twelve dogs with inflammatory bowel disease (IBD), dogs with IBD had lower TAC concentrations when compared with the healthy dogs. The method validated in this paper is precise, simple, and fast and can be easily adapted to automated analyzers.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Bathocuproinedisulfonic acid ; CUPRAC ; Dogs ; Oxidative stress ; Validation
Publicat a:	BMC veterinary research, Vol. 12 (july 2016) , ISSN 1746-6148

DOI: 10.1186/s12917-016-0760-2
PMID: 27370026

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