In Vivo, Non-Invasive Characterization of Human Bone by Hybrid Broadband (600-1200 nm) Diffuse Optical and Correlation Spectroscopies
Konugolu Venkata Sekar, Sanathana (Dipartimento di Fisica, Politecnico di Milano, Italy)
Pagliazzi, Marco (Institut de Ciència i Tecnologia de Barcelona)
Negredo Puigmal, Eugènia (Institut Germans Trias i Pujol. Fundació de Lluita Contra la Sida)
Martelli, Fabrizio (Università degli Studi di Firenze, Italy)
Farina, Andrea (Istituto di Fotonica e Nanotecnologie, Milano, Italy)
Dalla Mora, Alberto (Dipartimento di Fisica, Politecnico di Milano, Italy)
Lindner, Claus (Institut de Ciència i Tecnologia de Barcelona)
Farzam, Parisa (Institut de Ciència i Tecnologia de Barcelona)
Pérez-Álvarez, Núria (Institut Germans Trias i Pujol. Fundació de Lluita Contra la Sida)
Puig, Jordi (Institut Germans Trias i Pujol. Fundació de Lluita Contra la Sida)
Taroni, Paola (Dipartimento di Fisica, Politecnico di Milano, Italy)
Pifferi, Antonio (Dipartimento di Fisica, Politecnico di Milano, Italy)
Durduran, Turgut (Institució Catalana de Recerca i Estudis Avançats)

Date:	2016
Abstract:	Non-invasive in vivo diffuse optical characterization of human bone opens a new possibility of diagnosing bone related pathologies. We present an in vivo characterization performed on seventeen healthy subjects at six different superficial bone locations: radius distal, radius proximal, ulna distal, ulna proximal, trochanter and calcaneus. A tailored diffuse optical protocol for high penetration depth combined with the rather superficial nature of considered tissues ensured the effective probing of the bone tissue. Measurements were performed using a broadband system for Time-Resolved Diffuse Optical Spectroscopy (TRS) to assess mean absorption and reduced scattering spectra in the 600-1200 nm range and Diffuse Correlation Spectroscopy (DCS) to monitor microvascular blood flow. Significant variations among tissue constituents were found between different locations; with radius distal rich of collagen, suggesting it as a prominent location for bone related measurements, and calcaneus bone having highest blood flow among the body locations being considered. By using TRS and DCS together, we are able to probe the perfusion and oxygen consumption of the tissue without any contrast agents. Therefore, we predict that these methods will be able to evaluate the impairment of the oxygen metabolism of the bone at the point-of-care.
Grants:	European Commission 317526 Ministerio de Economía y Competitividad MTM2015-64465-C2-1-R
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 11 (december 2016) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0168426
PMID: 27997565

16 p, 1.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles