Web of Science: 48 citations, Scopus: 52 citations, Google Scholar: citations
Postnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implications
Muñoz-González, Sara (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Ruggli, Nicolas (Institute of Virology and immunology)
Rosell, Rosa (Generalitat de Catalunya. Departament d'Agricultura, Ramaderia, Pesca, Alimentació i Medi Natural)
Pérez, Lester Josue (Centro Nacional de Sanidad Agropecuaria)
Frías-Leuporeau, Maria Teresa (Centro Nacional de Sanidad Agropecuaria)
Fraile, Lorenzo (Universitat de Lleida. Departament de Ciència Animal)
Montoya, Maria (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Córdoba, Lorena (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Domingo, Mariano (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Ehrensperger, Felix (University Zürich. Institute of Veterinary Pathology)
Summerfield, Artur (Institute of Virology and immunology)
Ganges, Llilianne (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Date: 2015
Abstract: It is well established that trans-placental transmission of classical swine fever virus (CSFV) during mid-gestation can lead to persistently infected offspring. The aim of the present study was to evaluate the ability of CSFV to induce viral persistence upon early postnatal infection. Two litters of 10 piglets each were infected intranasally on the day of birth with low and moderate virulence CSFV isolates, respectively. During six weeks after postnatal infection, most of the piglets remained clinically healthy, despite persistent high virus titres in the serum. Importantly, these animals were unable to mount any detectable humoral and cellular immune response. At necropsy, the most prominent gross pathological lesion was a severe thymus atrophy. Four weeks after infection, PBMCs from the persistently infected seronegative piglets were unresponsive to both, specific CSFV and non-specific PHA stimulation in terms of IFN-γ-producing cells. These results suggested the development of a state of immunosuppression in these postnatally persistently infected pigs. However, IL-10 was undetectable in the sera of the persistently infected animals. Interestingly, CSFV-stimulated PBMCs from the persistently infected piglets produced IL-10. Nevertheless, despite the addition of the anti-IL-10 antibody in the PBMC culture from persistently infected piglets, the response of the IFN-γ producing cells was not restored. Therefore, other factors than IL-10 may be involved in the general suppression of the T-cell responses upon CSFV and mitogen activation. Interestingly, bone marrow immature granulocytes were increased and targeted by the virus in persistently infected piglets. Taken together, we provided the first data demonstrating the feasibility of CSFV in generating a postnatal persistent disease, which has not been shown for other members of the Pestivirus genus yet. Since serological methods are routinely used in CSFV surveillance, persistently infected pigs might go unnoticed. In addition to the epidemiological and economic significance of persistent CSFV infection, this model could be useful for understanding the mechanisms of viral persistence.
Grants: Agència de Gestió d'Ajuts Universitaris i de Recerca FI-DGR 2014
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: Article ; recerca ; Versió publicada
Subject: Swine fever
Published in: PloS one, Vol. 10 (may 2015) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0125692
PMID: 25938664


22 p, 5.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles

 Record created 2022-02-07, last modified 2022-10-07



   Favorit i Compartir