Interleukin-19 impairment in active Crohn's disease patients
Cantó, Elisabet (Institut d'Investigació Biomèdica Sant Pau)
García-Planella, Esther (Institut d'Investigació Biomèdica Sant Pau)
Zamora, Carlos (Institut d'Investigació Biomèdica Sant Pau)
Nieto Sáchica, Juan Camilo (Institut d'Investigació Biomèdica Sant Pau)
Gordillo, Jordi (Institut d'Investigació Biomèdica Sant Pau)
Ortiz, Ma Angels (Institut d'Investigació Biomèdica Sant Pau)
Metón, Isidoro (Universitat de Barcelona)
Serrano, Elena (Institut d'Investigació Biomèdica Sant Pau)
Vegas, Esteban (Universitat de Barcelona)
García-Bosch, Orlando (Hospital de Sant Joan Despí Moisès Broggi)
Juarez, Candido (Institut d'Investigació Biomèdica Sant Pau)
Vidal, Silvia (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Date:	2014
Abstract:	The exact function of interleukin-19 (IL-19) on immune response is poorly understood. In mice, IL-19 up-regulates TNFα and IL-6 expression and its deficiency increases susceptibility to DSS-induced colitis. In humans, IL-19 favors a Th2 response and is elevated in several diseases. We here investigate the expression and effects of IL-19 on cells from active Crohn's disease (CD) patient. Twenty-three active CD patients and 20 healthy controls (HC) were included. mRNA and protein IL-19 levels were analyzed in monocytes. IL-19 effects were determined in vitro on the T cell phenotype and in the production of cytokines by immune cells. We observed that unstimulated and TLR-activated monocytes expressed significantly lower IL-19 mRNA in active CD patients than in HC (logFC = −1. 97 unstimulated; −1. 88 with Pam3CSK4; and −1. 91 with FSL-1; p<0. 001). These results were confirmed at protein level. Exogenous IL-19 had an anti-inflammatory effect on HC but not on CD patients. IL-19 decreased TNFα production in PBMC (850. 7±75. 29 pg/ml vs 2626. 0±350 pg/ml; p<0. 01) and increased CTLA4 expression (22. 04±1. 55% vs 13. 98±2. 05%; p<0. 05) and IL-4 production (32. 5±8. 9 pg/ml vs 13. 5±2. 9 pg/ml; p<0. 05) in T cells from HC. IL-10 regulated IL-19 production in both active CD patients and HC. We observed that three of the miRNAs that can modulate IL-19 mRNA expression, were up-regulated in monocytes from active CD patients. These results suggested that IL-19 had an anti-inflammatory role in this study. Defects in IL-19 expression and the lack of response to this cytokine could contribute to inflammatory mechanisms in active CD patients.
Grants:	Ministerio de Economía, Industria y Competitividad PS09/132
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 9 (april 2014) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0093910
PMID: 24718601

10 p, 706.7 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles