Active Smoking Increases Microsomal PGE-Synthase-1/PGE-Receptor-4 Axis in Human Abdominal Aortic Aneurysms
Dilme, Jaime (Institut d'Investigació Biomèdica Sant Pau)
Solà-Villà, David (Institut d'Investigació Biomèdica Sant Pau)
Bellmunt Montoya, Sergi (Institut d'Investigació Biomèdica Sant Pau)
Romero, José María (Institut d'Investigació Biomèdica Sant Pau)
Escudero, José Román (Institut d'Investigació Biomèdica Sant Pau)
Camacho, Mercedes (Institut d'Investigació Biomèdica Sant Pau)
Vila, Luis (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Date:	2014
Abstract:	Background. The cyclooxygenase- (COX-) 2/microsomal PGE-synthase- (mPGES-) 1/PGE-receptor- (EP-) 4 axis could play a key role in the physiopathology of abdominal aortic aneurysm (AAA) in humans. In this study, we investigated the influence of cardiovascular risk factors on the expression of the PGE pathway in human AAA. Methods. Aortic (n = 89) and plasma (n = 79) samples from patients who underwent AAA repair were collected. Patients were grouped according to risk factors. COX-isoenzymes, mPGES-1, EPs, α -actin, and CD45 and CD68 transcripts levels were quantified by QRT-PCR and plasma PGE metabolites by EIA. Results. Current smoking (CS) patients compared to no-CS had significantly higher local levels of mPGES-1 (P = 0. 009), EP-4 (P = 0. 007), and PGE metabolites plasma levels (P = 0. 008). In the multiple linear regression analysis, these parameters remained significantly enhanced in CS after adding confounding factors. Results from association studies with cell type markers suggested that the increased mPGES-1/EP-4 levels were mainly associated with microvascular endothelial cells. Conclusions. This study shows that elements of the PGE pathway, which play an important role in AAA development, are increased in CS. These results provide insight into the relevance of tobacco smoking in AAA development and reinforce the potential of mPGES-1 and EP-4 as targets for therapy in AAA patients.
Grants:	Ministerio de Economía, Industria y Competitividad SAF2010/21392 Instituto de Salud Carlos III RD12/0042/0051
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Mediators of Inflammation, Vol. 2014 (april 2014) , ISSN 1466-1861

DOI: 10.1155/2014/316150
PMID: 24876670

11 p, 5.2 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles