Length and GC Content Variability of Introns among Teleostean Genomes in the Light of the Metabolic Rate Hypothesis
Chaurasia, Ankita (Centre de Recerca en Agrigenòmica)
Tarallo, Andrea (Stazione Zoologica Anton Dohrn. Department of Animal Physiology and Evolution)
Bernà, Luisa (Institut Pasteur de Montevideo. Molecular Biology Unit)
Yagi, Mitsuharu (Nagasaki University. Faculty of Fisheries)
Agnisola, Claudio (University of Naples Federico II. Department of Biological Sciences)
D'Onofrio, Giuseppe (Stazione Zoologica Anton Dohrn. Department of Animal Physiology and Evolution)

Data:	2014
Resum:	A comparative analysis of five teleostean genomes, namely zebrafish, medaka, three-spine stickleback, fugu and pufferfish was performed with the aim to highlight the nature of the forces driving both length and base composition of introns (i. e. , bpi and GCi). An inter-genome approach using orthologous intronic sequences was carried out, analyzing independently both variables in pairwise comparisons. An average length shortening of introns was observed at increasing average GCi values. The result was not affected by masking transposable and repetitive elements harbored in the intronic sequences. The routine metabolic rate (mass specific temperature-corrected using the Boltzmann's factor) was measured for each species. A significant correlation held between average differences of metabolic rate, length and GC content, while environmental temperature of fish habitat was not correlated with bpi and GCi. Analyzing the concomitant effect of both variables, i. e. , bpi and GCi, at increasing genomic GC content, a decrease of bpi and an increase of GCi was observed for the significant majority of the intronic sequences (from ∼40% to ∼90%, in each pairwise comparison). The opposite event, concomitant increase of bpi and decrease of GCi, was counter selected (from <1% to ∼10%, in each pairwise comparison). The results further support the hypothesis that the metabolic rate plays a key role in shaping genome architecture and evolution of vertebrate genomes.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 9, Num. 8 (August 2014) , art. e103889, ISSN 1932-6203

DOI: 10.1371/journal.pone.0103889
PMID: 25093416

11 p, 2.5 MB

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