Functional and Morphological Changes Induced in Mytilus Hemocytes by Selected Nanoparticles
Auguste, Manon (University of Genoa. Department of Environmental, Earth, and Life Sciences)
Mayall, Craig (Univerza V Ljubljani)
Barbero, Francesco (Institut Català de Nanociència i Nanotecnologia)
Hočevar, Matej (Institute of Metals and Technology)
Alberti, Stefano (University of Genoa. Department of Chemistry and Industrial Chemistry)
Grassi, Giacomo (University of Siena. Department of Physical, Earth, and Environmental Sciences)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Drobne, Damjana (Univerza V Ljubljani)
Canesi, Laura (University of Genoa. Department of Environmental, Earth, and Life Sciences)

Data:	2021
Resum:	Nanoparticles (NPs) show various properties depending on their composition, size, and surface coating, which shape their interactions with biological systems. In particular, NPs have been shown to interact with immune cells, that represent a sensitive surveillance system of external and internal stimuli. In this light, in vitro models represent useful tools for investigating nano-bio-interactions in immune cells of different organisms, including invertebrates. In this work, the effects of selected types of NPs with different core composition, size and functionalization (custom-made PVP-AuNP and commercial nanopolystyrenes PS-NH and PS-COOH) were investigated in the hemocytes of the marine bivalve Mytilus galloprovincialis. The role of exposure medium was evaluated using either artificial seawater (ASW) or hemolymph serum (HS). Hemocyte morphology was investigated by scanning electron microscopy (SEM) and different functional parameters (lysosomal membrane stability, phagocytosis, and lysozyme release) were evaluated. The results show distinct morphological and functional changes induced in mussel hemocytes depending on the NP type and exposure medium. Mussel hemocytes may represent a powerful alternative in vitro model for a rapid pre-screening strategy for NPs, whose utilization will contribute to the understanding of the possible impact of environmental exposure to NPs in marine invertebrates.
Ajuts:	European Commission 671881
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Hemocytes ; Mytilus ; In vitro ; Scanning electron microscopy ; Immune response
Publicat a:	Nanomaterials, Vol. 11, Issue 2 (February 2021) , art. 470, ISSN 2079-4991

DOI: 10.3390/nano11020470
PMID: 33673220

16 p, 8.0 MB

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