Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry and High-Sensitivity Gas Chromatography-Mass Spectrometry Screening of Classic Drugs and New Psychoactive Substances and Metabolites in Urine of Consumers
Marchei, Emilia (Istituto Superiore di Sanità (Itàlia))
Alías Ferri, María (Universitat Autònoma de Barcelona. Departament de Farmacologia, de Terapèutica i de Toxicologia)
Torrens, Marta (Universitat Autònoma de Barcelona. Departament de Farmacologia, de Terapèutica i de Toxicologia)
Farre, Magi (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pacifici, Roberta (Istituto Superiore di Sanità (Itàlia))
Pichini, Simona (Istituto Superiore di Sanità (Itàlia))
Pellegrini, Manuela (Istituto Superiore di Sanità (Itàlia))

Data:	2021
Resum:	The use of the new psychoactive substances is continuously growing and the implementation of accurate and sensible analysis in biological matrices of users is relevant and fundamental for clinical and forensic purposes. Two different analytical technologies, high-sensitivity gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) were used for a screening analysis of classic drugs and new psychoactive substances and their metabolites in urine of formed heroin addicts under methadone maintenance therapy. Sample preparation involved a liquid-liquid extraction. The UHPLC-HRMS method included Accucore™ phenyl Hexyl (100 × 2. 1 mm, 2. 6 μm, Thermo, USA) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2 mM in water, 0. 1% formic acid) and mobile phase B (ammonium formate 2 mM in methanol/acetonitrile 50:50 (v / v), 0. 1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode for substances identification (mass range 100-1000 m / z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m, 250 µm i. d, film thickness 0. 25 µm; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40-550 m / z). Urine samples from 296 patients with a history of opioid use disorder were examined. Around 80 different psychoactive substances and/or metabolites were identified, being methadone and metabolites the most prevalent ones. The possibility to screen for a huge number of psychotropic substances can be useful in suspected drug related fatalities or acute intoxication/exposure occurring in emergency departments and drug addiction services.
Ajuts:	European Commission. Horizon 2020 806996 Instituto de Salud Carlos III PI14/00715 Instituto de Salud Carlos III PI17/01962 Instituto de Salud Carlos III RD16/0017/0003 Instituto de Salud Carlos III RD16/0017/0010 Generalitat de Catalunya 2017SGR316 Generalitat de Catalunya 2017SGR530
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Classic drugs of abuse ; New psychoactive substances (NPS) ; Novel synthetic opioids (NSO) ; Urine ; Liquid chromatography ; High-resolution mass spectrometry ; Gas chromatography-mass spectrometry
Publicat a:	International journal of molecular sciences, Vol. 22 (april 2021) , ISSN 1422-0067

DOI: 10.3390/ijms22084000
PMID: 33924438

21 p, 1.9 MB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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