Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
Salomón, Ricardo (Universitat Autònoma de Barcelona)
Reyes-López, Felipe E. (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Firmino, Joana P. (Universitat Autònoma de Barcelona)
Sarasquete, Carmen (Universidad de Cádiz)
Ortiz-Delgado, Juan B. (Universidad de Cádiz)
Quintela, José C. (Natac Biotech)
Pinilla-Rosas, José M. (Natac Biotech)
Vallejos Vidal, Eva Carolina (Universidad de Santiago de Chile)
Gisbert, Enric (Institut de Recerca i Tecnologia Agroalimentàries)

Data:	2021
Resum:	The inclusion of a medicinal plant leaf extract (MPLE) from sage (Salvia officinalis) and lemon verbena (Lippia citriodora), rich in verbascoside and triterpenic compounds like ursolic acid, was evaluated in gilthead seabream (Sparus aurata) fed a low fishmeal-based diet (48% crude protein, 17% crude fat, 21. 7 MJ kg -1, 7% fishmeal, 15% fish oil) for 92 days. In particular, the study focused on the effect of these phytogenic compounds on the gut condition by analyzing the transcriptomic profiling (microarray analysis) and histological structure of the intestinal mucosa, as well as the histochemical properties of mucins stored in goblet cells. A total number of 506 differentially expressed genes (285 up- and 221 down-regulated) were found when comparing the transcriptomic profiling of the intestine from fish fed the control and MPLE diets. The gut transcripteractome revealed an expression profile that favored biological mechanisms associated to the 1) immune system, particularly involving T cell activation and differentiation, 2) gut integrity (i. e. , adherens and tight junctions) and cellular proliferation, and 3) cellular proteolytic pathways. The histological analysis showed that the MPLE dietary supplementation promoted an increase in the number of intestinal goblet cells and modified the composition of mucins' glycoproteins stored in goblet cells, with an increase in the staining intensity of neutral mucins, as well as in mucins rich in carboxylated and weakly sulfated glycoconjugates, particularly those rich in sialic acid residues. The integration of transcriptomic and histological results showed that the evaluated MPLE from sage and lemon verbena is responsible for the maintenance of intestinal health, supporting gut homeostasis and increasing the integrity of the intestinal epithelium, which suggests that this phytogenic may be considered as a promising sustainable functional additive for aquafeeds.
Ajuts:	European Commission 727315 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/DI-017
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cell proliferation ; Feed additive ; Gut health ; Innate immunity ; Lectin histochemistry ; Ursolic acid ; Verbascoside acid ; GALT
Publicat a:	Frontiers in immunology, Vol. 12 (May 2021) , art. 670279, ISSN 1664-3224

DOI: 10.3389/fimmu.2021.670279
PMID: 34054843

18 p, 4.0 MB

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