An in vitro protocol to study the modulatory effects of a food or biocompound on human gut microbiome and metabolome
Rosés, Carles (Universitat Autònoma de Barcelona. Servei de Genòmica)
Nieto, Juan Antonio (Parque Tecnológico de Valencia)
Viadel, Blanca (Parque Tecnológico de Valencia)
Gallego, Elisa (Parque Tecnológico de Valencia)
Romo-Hualde, Ana (Universidad de Navarra)
Streitenberger, Sergio (AMC Innova)
Milagro, Fermin I (Universidad de Navarra)
Barceló Vernet, Anna (Universitat Autònoma de Barcelona. Servei de Genòmica)

Data:	2021
Resum:	The gut microbiota plays a key role in gastrointestinal immune and metabolic functions and is influenced by dietary composition. An in vitro protocol simulating the physiological conditions of the digestive system helps to study the effects of foods/biocompounds on gut microbiome and metabolome. The Dynamic-Colonic Gastrointestinal Digester consists of five interconnected compartments, double jacket vessels that simulate the physiological conditions of the stomach, the small intestine and the three colonic sections, which are the ascending colon, transverse colon and descending colon. Human faeces are required to reproduce the conditions and culture medium of the human colon, allowing the growth of the intestinal microbiota. After a stabilization period of 12 days, a food/biocompound can be introduced to study its modulatory effects during the next 14 days (treatment period). At the end of the stabilization and treatment period, samples taken from the colon compartments are analysed. The 16S rRNA gene analysis reveals the microbiota composition. The untargeted metabolomics analysis gives more than 10,000 features (metabolites/compounds). The present protocol allows in vitro testing of the modulatory effects of foods or biocompounds on gut microbiota composition and metabolic activity.
Ajuts:	Ministerio de Economía y Competitividad CB12/03/30002
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Microbiota ; Digestor ; Food compounds ; Colonic fermentation ; Metabolome
Publicat a:	Foods, Vol. 10, Núm. 12 (December 2021) , art. 3020, ISSN 2304-8158

DOI: 10.3390/foods10123020
PMID: 34945571

14 p, 2.0 MB

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