Insights into the light response of skeletonema marinoi : Involvement of ovothiol
Milito, Alfonsina (Centre de Recerca en Agrigenòmica)
Orefice, Ida (Stazione Zoologica Anton Dohrn. Department of Marine Biotechnology)
Smerilli, Arianna (Stazione Zoologica Anton Dohrn. Department of Marine Biotechnology)
Castellano, Immacolata (Stazione Zoologica Anton Dohrn. Department of Biology and Evolution of Marine Organisms)
Napolitano, Alessandra (University of Naples "Federico II". Department of Chemical Sciences)
Brunet, Christophe (Stazione Zoologica Anton Dohrn. Department of Marine Biotechnology)
Palumbo, Anna (Stazione Zoologica Anton Dohrn. Department of Biology and Evolution of Marine Organisms)

Data:	2020
Resum:	Diatoms are one of the most widespread groups of microalgae on Earth. They possess extraordinary metabolic capabilities, including a great ability to adapt to different light conditions. Recently, we have discovered that the diatom Skeletonema marinoi produces the natural antioxidant ovothiol B, until then identified only in clams. In this study, we investigated the light-dependent modulation of ovothiol biosynthesis in S. marinoi. Diatoms were exposed to different light conditions, ranging from prolonged darkness to low or high light, also differing in the velocity of intensity increase (sinusoidal versus square-wave distribution). The expression of the gene encoding the key ovothiol biosynthetic enzyme, ovoA, was upregulated by high sinusoidal light mimicking natural conditions. Under this situation higher levels of reactive oxygen species and nitric oxide as well as ovothiol and glutathione increase were detected. No ovoA modulation was observed under prolonged darkness nor low sinusoidal light. Unnatural conditions such as continuous square-wave light induced a very high oxidative stress leading to a drop in cell growth, without enhancing ovoA gene expression. Only one of the inducible forms of nitric oxide synthase, nos2, was upregulated by light with consequent production of NO under sinusoidal light and darkness conditions. Our data suggest that ovothiol biosynthesis is triggered by a combined light stress caused by natural distribution and increased photon flux density, with no influence from the daily light dose. These results open new perspectives for the biotechnological production of ovothiols, which are receiving a great interest for their biological activities in human model systems.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Algae ; Antioxidant ; Diatoms ; Light ; Nitric oxide ; Ovothiol ; Oxidative stress
Publicat a:	Marine drugs, Vol. 18, Núm. 9 (September 2020) , art. 477, ISSN 1660-3397

DOI: 10.3390/md18090477
PMID: 32962291

18 p, 2.0 MB

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