Medicated scaffolds prepared with Hydroxyapatite/Streptomycin nanoparticles encapsulated into Polylactide microfibers
Kadkhodaie-Elyaderani, Amirmajid (Universitat Politècnica de Catalunya. Departament d'Enginyeria Química)
de Lama-Odría, Maria del Carmen (Universitat Politècnica de Catalunya. Centre de Recerca en Ciencia i Enginyeria Multiescala de Barcelona)
Rivas, Manuel (Universitat Politècnica de Catalunya. Centre de Recerca en Ciencia i Enginyeria Multiescala de Barcelona)
Martínez-Rovira, Immaculada (ALBA Laboratori de Llum de Sincrotró)
Yousef, Ibraheem (ALBA Laboratori de Llum de Sincrotró)
Puiggalí, Jordi (Institut de Bioenginyeria de Catalunya)
del Valle, Luis J. (Universitat Politècnica de Catalunya. Centre de Recerca en Ciencia i Enginyeria Multiescala de Barcelona)

Data:	2022
Resum:	The preparation, characterization, and controlled release of hydroxyapatite (HAp) nanoparticles loaded with streptomycin (STR) was studied. These nanoparticles are highly appropriate for the treatment of bacterial infections and are also promising for the treatment of cancer cells. The analyses involved scanning electron microscopy, dynamic light scattering (DLS) and Z-potential measurements, as well as infrared spectroscopy and X-ray diffraction. Both amorphous (ACP) and crystalline (cHAp) hydroxyapatite nanoparticles were considered since they differ in their release behavior (faster and slower for amorphous and crystalline particles, respectively). The encapsulated nanoparticles were finally incorporated into biodegradable and biocompatible polylactide (PLA) scaffolds. The STR load was carried out following different pathways during the synthesis/precipitation of the nanoparticles (i. e. , nucleation steps) and also by simple adsorption once the nanoparticles were formed. The loaded nanoparticles were biocompatible according to the study of the cytotoxicity of extracts using different cell lines. FTIR microspectroscopy was also employed to evaluate the cytotoxic effect on cancer cell lines of nanoparticles internalized by endocytosis. The results were promising when amorphous nanoparticles were employed. The nanoparticles loaded with STR increased their size and changed their superficial negative charge to positive. The nanoparticles' crystallinity decreased, with the consequence that their crystal sizes reduced, when STR was incorporated into their structure. STR maintained its antibacterial activity, although it was reduced during the adsorption into the nanoparticles formed. The STR release was faster from the amorphous ACP nanoparticles and slower from the crystalline cHAp nanoparticles. However, in both cases, the STR release was slower when incorporated in calcium and phosphate during the synthesis. The biocompatibility of these nanoparticles was assayed by two approximations. When extracts from the nanoparticles were evaluated in cultures of cell lines, no cytotoxic damage was observed at concentrations of less than 10 mg/mL. This demonstrated their biocompatibility. Another experiment using FTIR microspectroscopy evaluated the cytotoxic effect of nanoparticles internalized by endocytosis in cancer cells. The results demonstrated slight damage to the biomacromolecules when the cells were treated with ACP nanoparticles. Both ACP and cHAp nanoparticles were efficiently encapsulated in PLA electrospun matrices, providing functionality and bioactive properties.
Ajuts:	Agencia Estatal de Investigación RTI2018-101827-B-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-373 Ministerio de Ciencia e Innovación RYC2018-024043-I
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Hydroxyapatite nanoparticles ; Streptomycin ; Polylactide ; Drug delivery ; Antimicrobial activity ; Cytotoxicity
Publicat a:	International journal of molecular sciences, Vol. 23, Issue 3 (February 2022) , art. 1282, ISSN 1422-0067

DOI: 10.3390/ijms23031282
PMID: 35163204

27 p, 4.1 MB

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