Automated parasitological diagnosis in clinical microbiology laboratories
Fernandez Rivas, Gema (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rivaya, Belén (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Romani Rodes, Nona (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Wang-Wang, Jun Hao (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Alcaide, Mireya (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Matas, Lurdes (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)

Data:	2021
Resum:	Although there is a low prevalence of parasitological infections in Europe, the diagnosis of intestinal parasites is still difficult and laborious for microbiology laboratories. Currently, antigen detection assays and molecular biology allow a more accurate diagnosis, but these techniques have limitations as they cannot detect all the possible parasites present in the samples. The objective of the study was to evaluate the accuracy and the usefulness of automated microscopy SediMAX2 (77 Elektronika, Budapest, Hungary) in the detection of parasitic infections from feces. A total of 197 formol-fixed stool samples were processed in parallel by wet mount examination and by SediMAX2. Sensitivities, specificities and predictive values were analyzed, reaching a sensitivity of 89. 51% and a specificity of 98. 15% and a very good positive predictive value (99. 22%). SediMAX2 is a good tool for a reliable diagnosis of intestinal parasitic infections. The rapid processing and the flexibilty of storage of images analyzed make its incorporation into the day to day laboratory routine recommendable.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Infectious diseases ; Microbiology ; Clinical microbiology ; Infectious-disease diagnostics ; Parasitology
Publicat a:	Scientific reports, Vol. 11 (june 2021) , ISSN 2045-2322

DOI: 10.1038/s41598-021-92441-3
PMID: 34162917

6 p, 1.1 MB

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