Humoral Responses and Ex Vivo IFN-γ Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein
Martínez-Orellana, Pamela (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
González, Noemí (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Baldassarre, Antonella (Università degli Studi di Bari Aldo Moro. Dipartimento delle Emergenze e dei Trapianti d' Organo)
Álvarez-Fernández, Alejandra (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ordeix, Laura (Ordeix i Esteve) (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Paradies, Paola (Università degli Studi di Bari Aldo Moro. Dipartimento delle Emergenze e dei Trapianti d' Organo)
Soto, Manuel (Universidad Autónoma de Madrid, Departamento de Biología Molecular)
Solano Gallego, Laia (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Date:	2022
Abstract:	The effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN-γ (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided into Group 1: healthy (Asturias, Spain, n = 26), Group 2: sick (n = 46), Group 3: healthy Ibizan hounds (Mallorca, Spain, n = 22) and Group 4: healthy (Bari, Italy, n = 14). Antibody levels were higher for LSA when compared to rKMP11 (p = 0. 001). Ibizan hounds were all seronegative to rKMP11 and 18% were low seropositive to LSA. Sick dogs presented higher antibody response to both antigens compared to the rest of the groups (p < 0. 0001). All groups showed higher IFN-γ levels after LSA compared to rKMP11 responses (p < 0. 05). The highest response to LSA was found in Ibizan hounds (p < 0. 05). IFN-γ to LSA and rKMP11 stimulation was observed in 34% and in 2. 8% of the sick dogs, respectively. Here, we demonstrated that anti-rKMP11 antibodies are mainly present in dogs with moderate to severe disease. Furthermore, cellular immune response measured by specific ex vivo IFN-γ production was more intense to LSA than stimulated to rKMP11.
Grants:	Ministerio de Economía y Competitividad AGL2015-68477
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; Versió publicada
Subject:	Leishmaniosis ; Serology ; Dog ; Cell-mediated immunity ; Diagnosis
Published in:	Veterinary sciences, Vol. 9 Núm. 3 (2022) , p. 116, ISSN 2306-7381

DOI: 10.3390/vetsci9030116
PMID: 35324844

10 p, 8.9 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Published articles