CRISPR/Cas9 gene editing uncovers the roles of CONSTITUTIVE TRIPLE RESPONSE 1 and REPRESSOR OF SILENCING 1 in melon fruit ripening and epigenetic regulation
Giordano, Andrea (Centre de Recerca en Agrigenòmica)
Santo Domingo, Miguel (Centre de Recerca en Agrigenòmica)
Quadrana, Leandro (Institut de Biologie de l'Ecole Normale Supérieure)
Pujol Abajo, Marta (Centre de Recerca en Agrigenòmica)
Martín-Hernández, Ana Montserrat (Centre de Recerca en Agrigenòmica)
Garcia-Mas, Jordi (Centre de Recerca en Agrigenòmica)

Date:	2022
Abstract:	Melon (Cucumis melo) has emerged as an alternative model to tomato for studying fruit ripening due to the coexistence of climacteric and non-climacteric varieties. Previous characterization of a major quantitative trait locus (QTL), ETHQV8. 1, that is able to trigger climacteric ripening in a non-climacteric background resulted in the identification of a negative regulator of ripening CTR1-like (MELO3C024518) and a putative DNA demethylase ROS1 (MELO3C024516) that is the orthologue of DML2, a DNA demethylase that regulates fruit ripening in tomato. To understand the role of these genes in climacteric ripening, in this study we generated homozygous CRISPR knockout mutants of CTR1-like and ROS1 in a climacteric genetic background. The climacteric behavior was altered in both loss-of-function mutants in two growing seasons with an earlier ethylene production profile being observed compared to the climacteric wild type, suggesting a role of both genes in climacteric ripening in melon. Single-cytosine methylome analyses of the ROS1-knockout mutant revealed changes in DNA methylation in the promoter regions of the key ripening genes such as ACS1, ETR1, and ACO1, and in transcription factors associated with ripening including NAC-NOR, RIN, and CNR, suggesting the importance of ROS1-mediated DNA demethylation for triggering fruit ripening in melon.
Grants:	Agencia Estatal de Investigación RTI2018-097665-B-C2 Agencia Estatal de Investigación CEX2019-000902 European Commission 793090 Ministerio de Economía, Industria y Competitividad BES-2017-079956 European Commission 948674
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Published in:	Journal of Experimental Botany, Vol. 73, Issue 12 (June 2022) , p. 4022-4033, ISSN 0022-0957

DOI: 10.1093/jxb/erac148

Preprint 25 p, 23.2 MB

Postprint 24 p, 23.9 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles