Study of the variation of the Malassezia load in the interdigital fold of dogs with pododermatitis
Díaz Álvarez, Leyna (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Castellá, Gemma (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Bragulat, M. Rosa (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Paytuví-Gallart, Andreu (Sequentia Biotech SL)
Sanseverino, Walter (Sequentia Biotech SL)
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Data:	2022
Resum:	The yeast Malassezia pachydermatis is a common inhabitant of the skin and mucosae of dogs. However, under certain circumstances this yeast can overgrow and act as an opportunistic pathogen causing otitis and dermatitis in dogs. Canine pododermatitis is a common disorder in dogs in which M. pachydermatis acts as an opportunistic pathogen. In the present study, the presence of Malassezia yeasts was assessed and quantified in samples collected from the interdigital space of dogs with pododermatitis before and after treatment, and from healthy dogs. The samples were subjected to two different cytological examinations, culture on Sabouraud glucose agar and modified Dixon's agar and a quantitative PCR targeting the internal transcribed spacer (ITS) genomic region. A selection of samples was analyzed by next generation sequencing (NGS) using the D1D2 domain of the large subunit of the ribosomal DNA as target. The pododermatitis samples before treatment showed higher cell counts, colony-forming units and ITS copies than the rest of samples. The NGS analysis revealed that Ascomycota was the main phylum in the healthy and post-treatment samples. However, Basidiomycota and M. pachydermatis was more abundant in the pododermatitis samples before treatment. These results support M. pachydermatis as an opportunistic agent in canine pododermatitis by a variety of methods, and demonstrate the correlation between cytologic and molecular methods for quantification.
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Interdigital fold ; Malassezia ; M. pachydermatis ; Mycobiome ; Pododermatitis
Publicat a:	Veterinary Research Communications, (June 2022) , ISSN 1573-7446

DOI: 10.1007/s11259-022-09951-2
PMID: 35704160

12 p, 2.7 MB

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