Nanoscale Wetting of Single Viruses
Calò, Annalisa (Universitat de Barcelona. Departament d'Enginyeria Electrònica i Biomèdica)
Eleta-Lopez, Aitziber (CIC nanoGUNE (Sant Sebastià, País Basc))
Ondarçuhu, Thierry (Université de Toulouse. Institut de Mécanique des Fluides de Toulouse)
Verdaguer Prats, Albert (Institut Català de Nanociència i Nanotecnologia)
Bittner, Alexander M. (Ikerbasque. Fundació Basca per a la Ciència)

Date:	2021
Abstract:	The epidemic spread of many viral infections is mediated by the environmental conditions and influenced by the ambient humidity. Single virus particles have been mainly visualized by atomic force microscopy (AFM) in liquid conditions, where the effect of the relative humidity on virus topography and surface cannot be systematically assessed. In this work, we employed multi-frequency AFM, simultaneously with standard topography imaging, to study the nanoscale wetting of individual Tobacco Mosaic virions (TMV) from ambient relative humidity to water condensation (RH > 100%). We recorded amplitude and phase vs. distance curves (APD curves) on top of single virions at various RH and converted them into force vs. distance curves. The high sensitivity of multifrequency AFM to visualize condensed water and sub-micrometer droplets, filling gaps between individual TMV particles at RH > 100%, is demonstrated. Dynamic force spectroscopy allows detecting a thin water layer of thickness ~1 nm, adsorbed on the outer surface of single TMV particles at RH < 60%.
Grants:	Ministerio de Economía y Competitividad MAT2013-4006-R Agencia Estatal de Investigación PID2019-104650GB-C22 Ministerio de Economía y Competitividad MDM-2016-0618
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Tobacco Mosaic Virus (TMV) ; Nanoscale wetting ; Multifrequency AFM ; Force reconstruction ; Amplitude-modulation AM-AFM
Published in:	Molecules, Vol. 26, Issue 17 (September 2021) , art. 5184, ISSN 1420-3049

DOI: 10.3390/molecules26175184
PMID: 34500617

11 p, 2.9 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles