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Pàgina inicial > Articles > Articles publicats > Nanoscale Wetting of Single Viruses |
Data: | 2021 |
Resum: | The epidemic spread of many viral infections is mediated by the environmental conditions and influenced by the ambient humidity. Single virus particles have been mainly visualized by atomic force microscopy (AFM) in liquid conditions, where the effect of the relative humidity on virus topography and surface cannot be systematically assessed. In this work, we employed multi-frequency AFM, simultaneously with standard topography imaging, to study the nanoscale wetting of individual Tobacco Mosaic virions (TMV) from ambient relative humidity to water condensation (RH > 100%). We recorded amplitude and phase vs. distance curves (APD curves) on top of single virions at various RH and converted them into force vs. distance curves. The high sensitivity of multifrequency AFM to visualize condensed water and sub-micrometer droplets, filling gaps between individual TMV particles at RH > 100%, is demonstrated. Dynamic force spectroscopy allows detecting a thin water layer of thickness ~1 nm, adsorbed on the outer surface of single TMV particles at RH < 60%. |
Ajuts: | Ministerio de Economía y Competitividad MAT2013-4006-R Agencia Estatal de Investigación PID2019-104650GB-C22 Ministerio de Economía y Competitividad MDM-2016-0618 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Tobacco Mosaic Virus (TMV) ; Nanoscale wetting ; Multifrequency AFM ; Force reconstruction ; Amplitude-modulation AM-AFM |
Publicat a: | Molecules, Vol. 26, Issue 17 (September 2021) , art. 5184, ISSN 1420-3049 |
11 p, 2.9 MB |