Analysis of γ-rays induced chromosome aberrations : A fingerprint evaluation with a combination of pan-centromeric and pan-telomeric probes
Benkhaled, L. (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Barrios, Leonardo (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Mestres, M. (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Caballín, M. R. (María Rosa) (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Ribas, M. (Institut d'Investigació Biomèdica Sant Pau)
Barquinero, Joan Francesc (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)

Data:	2006
Resum:	Purpose: To evaluate the types of induced chromosome aberrations after the exposure of peripheral blood to γ-rays by the simultaneous detection of all centromeres and telomeres; and to analyse the suitability of different radiation fingerprints for the assessment of radiation quality in cases of recent exposures. Material and methods: Peripheral blood samples were irradiated at 2, 4 and 6 Gy of γ-rays. Cytogenetic analysis was carried out by fluorescence in situ hybridization (FISH) technique with pan-centromeric and peptide nucleic acid (PNA)-telomeric DNA probes. Cells were analysed using a Cytovision® FISH workstation, chromosome aberrations and the length of the acentric fragments were recorded. Results: The total number of the incomplete chromosome elements was 276. The ratio between incomplete elements and multicentrics was 0. 38. The number of acentrics was 1096, 71% were complete acentrics, 15% incomplete acentrics, and 14% interstitial fragments. The relative length of complete, incomplete and interstitial acentrics fragments were 2. 70 ± 0. 04, 1. 91 ± 0. 07, and 1. 42 ± 0. 04 respectively. The mean value of the F-ratio was 11. 5 higher than the one, 5. 5, previously obtained for α-particles. For the G-ratio there was no difference between γ-rays and α-particles, 2. 8 and 2. 8 respectively. The mean value of the H-ratio for γ-rays, 0. 25, was lower than for α-particles 0. 40. Conclusion: The results support that the percentage of incomplete chromosome aberrations depends on radiation type; low-linear energy transfer (LET) radiation would produces less incomplete aberrations than high-LET radiation. The F- and H-ratios seem to be good indicators of radiation quality, although a real estimation of the H-ratio is only possible using pan-telomeric probes.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2001/SGR-00213
Nota:	Altres ajuts: This research received financial support from the Spanish "Consejo de Seguridad Nuclear" (SPR/04/4550)
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Acentric fragments ; Incomplete chromosome aberrations ; Pan-telomeric and pan-centromeric DNA probes ; Radiation quality fingerprints
Publicat a:	International Journal of Radiation Biology, Vol. 82, Issue 12 (2006) , p. 869-875, ISSN 0955-3002

DOI: 10.1080/09553000600979092

Postprint 24 p, 305.0 KB

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