Comparison of X-ray dose-response curves obtained by chromosome painting using conventional and PAINT nomenclatures
Barquinero, Joan Francesc (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Cigarrán Valea, Sergio (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Caballín, M. R. (María Rosa) (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Braselmann, H. (GSF, National Research Center for Environment and Health, Institute of Radiobiology)
Ribas, Montserrat (Institut d'Investigació Biomèdica Sant Pau)
Egozcue, Josep 1940-2006 (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Barrios, Leonardo (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Date:	1999
Abstract:	Purpose: The compare the suitability of PAINT and conventional nomenclature systems for the construction of chromosome aberration dose- effect curves for X-rays using FISH techniques, and to compare these curves with those based on solid-stained dicentrics analysed in first division metaphases by the FPG technique. Materials and methods: Blood samples were irradiated at 0. 1, 0. 25, 0. 50, 0. 75, 1, 1. 5, 2, 3, 4 and 5 Gy 180 kV X-rays. FISH painting was performed using probes for chromosomes 1, 4 and 11 in combination with a pan-centromeric probe. Results: Translocations showed a higher background frequency than dicentrics. This influences the ratio of translocations:dicentrics at the lower doses and the uncertainties of dose- effect curves for translocations. The dose-effect curves for dicentrics obtained by FISH and solid stain were in close agreement. Conclusion: For short-term biological dosimetry purposes by FISH, the use of dic(BA) (PAINT nomenclature) or total dicentrics (conventional nomenclature) should give similar dose estimates. For dose reconstruction, the use of total or complete translocations result in similar uncertainties.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 1998/SGR-00073
Note:	Altres ajuts: Consejo Español de Seguridad Nuclear (exp. 246/96)
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Radiation-induced chromosome aberrations ; Dose-response curves ; Solid stain ; FISH-painting ; PAINT and conventional nomenclatures
Published in:	International Journal of Radiation Biology, Vol. 75 Núm. 12 (1999) , p. 1557-1566, ISSN 0955-3002

DOI: 10.1080/095530099139160

38 p, 835.8 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles